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Volume 63, Issue Pt_11

sp. nov., a marine bacterium isolated from a tidal flat Free

Abstract

A Gram-negative, strictly aerobic, motile bacterium with flagella, designated strain G210, was isolated from a crude-oil-contaminated tidal flat of the Taean coast in South Korea. Cells were non-spore-forming, ovoid rods showing catalase- and oxidase-positive reactions. Growth of strain G210 was observed between 15 and 45 °C (optimum, 30–35 °C) and between pH 5.5 and 9.0 (optimum, pH 6.5–7.5). Strain G210 contained Q-10 as the sole isoprenoid quinone and summed feature 8 (Cω7 and/or Cω6) and C cyclo ω8 as the major fatty acids. Putrescine and spermidine were identified as the major polyamines. Strain G210 contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, an unidentified phospholipid and an unidentified aminolipid as polar lipids. The G+C content of the genomic DNA was 56.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G210 formed a phyletic lineage with members of the genus . Strain G210 was most closely related to 905/1 (97.9 % similarity). The DNA–DNA relatedness between strain G210 and the type strain of was 24±0.5 %. On the basis of phenotypic, chemotaxonomic and molecular properties, strain G210 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is G210 ( = KACC 14931 = JCM 16876).

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Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2012R1A1B)
  • Korean Government (MEST)
  • Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR), Korea
© 2013 IUMS
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2013-11-01
2024-04-23
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Aquamicrobium aestuarii sp. nov., a marine bacterium isolated from a tidal flat
Int J Syst Evol Microbiol 63, 4012 (2013); https://doi.org/10.1099/ijs.0.048561-0
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