1887

Abstract

Twenty -like isolates were obtained from environmental samples collected on a cattle ranch in northern Colorado; all of these isolates were found to share an identical partial sequence, suggesting close relatedness. The isolates were similar to members of the genus in that they were Gram-stain-positive, short rods, oxidase-negative and catalase-positive; the isolates were similar to because they were non-motile at 25 °C. 16S rRNA gene sequencing for representative isolates and whole genome sequencing for one isolate was performed. The genome of the type strain of (strain LU2006-1) was also sequenced. The draft genomes were very similar in size and the average MUMmer nucleotide identity across 91 % of the genomes was 95.16 %. Genome sequence data were used to design primers for a six-gene multi-locus sequence analysis (MLSA) scheme. Phylogenies based on (i) the near-complete 16S rRNA gene, (ii) 31 core genes and (iii) six housekeeping genes illustrated the close relationship of these -like isolates to LU2006-1. Sufficient genetic divergence of the -like isolates from the type strain of and differing phenotypic characteristics warrant these isolates to be classified as members of a distinct infraspecific taxon, for which the name subsp. subsp. nov. is proposed. The type strain is TTU M1-001 ( = BAA-2414 = DSM 25391). The isolates of subsp. subsp. nov. differ from the nominate subspecies by the inability to utilize melezitose, turanose and sucrose, and the ability to utilize inositol. The results also demonstrate the utility of whole genome sequencing to facilitate identification of novel taxa within a well-described genus. The genomes of both subspecies of contained putative enhancin genes; the subsp. subsp. nov. genome also encoded a putative mosquitocidal toxin. The presence of these genes suggests possible adaptation to an insect host, and further studies are needed to probe niche adaptation of .

Funding
This study was supported by the:
  • US Department of Agriculture, Cooperative State Research, Education and Extension Service (Award 2008-51110-04333)
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2013-09-01
2024-03-29
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