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Volume 63, Issue Pt_11

sp. nov., isolated from root nodules Free

Abstract

Two strains of Gram-stain-negative, rod-shaped bacteria were isolated from root nodules of the South African legume and authenticated on this host. Based on phylogenetic analysis of the 16S rRNA gene, strains WSM3930 and WSM3937 belonged to the genus , with the highest degree of sequence similarity to (98.84 %). Additionally, the housekeeping genes and were analysed since 16S rRNA gene sequences are highly similar between closely related species of the genus . The results obtained for both housekeeping genes, and , showed the highest degree of sequence similarity of the novel strains towards LMG 19076 (94.2 % and 94.5 %, respectively). Chemotaxonomic data, including fatty acid profiles and respiratory quinone data supported the assignment of strains WSM3930 and WSM3937 to the genus . DNA–DNA hybridizations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains WSM3930 and WSM3937 from the most closely related species of the genus with validly published names. We conclude, therefore, that these strains represent a novel species for which the name sp. nov. is proposed, with strain WSM3937 ( = LMG 27174 = HAMBI 3354) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • Australian Centre for International Agricultural Research
© 2013 IUMS
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2013-11-01
2024-04-25
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Burkholderia rhynchosiae sp. nov., isolated from Rhynchosia ferulifolia root nodules
Int J Syst Evol Microbiol 63, 3944 (2013); https://doi.org/10.1099/ijs.0.048751-0
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