RT Journal Article SR Electronic(1) A1 De Meyer, Sofie E. A1 Cnockaert, Margo A1 Ardley, Julie K. A1 Maker, Garth A1 Yates, Ron A1 Howieson, John G. A1 Vandamme, PeterYR 2013 T1 Burkholderia sprentiae sp. nov., isolated from Lebeckia ambigua root nodules JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_11 SP 3950 OP 3957 DO https://doi.org/10.1099/ijs.0.048777-0 PB Microbiology Society, SN 1466-5034, AB Seven Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Burkholderia , with the representative strain WSM5005T being most closely related to Burkholderia tuberum (98.08 % sequence similarity). Additionally, these strains formed a distinct group in phylogenetic trees based on the housekeeping genes gyrB and recA. Chemotaxonomic data including fatty acid profiles and analysis of respiratory quinones supported the assignment of the strains to the genus Burkholderia . Results of DNA–DNA hybridizations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from the closest species of the genus Burkholderia with a validly published name. Therefore, these strains represent a novel species for which the name Burkholderia sprentiae sp. nov. (type strain WSM5005T = LMG 27175T = HAMBI 3357T) is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.048777-0