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Volume 63, Issue Pt_7

sp. nov. and sp. nov., two actinobacteria isolated from grass soil Free

Abstract

Two strains, PB34 and PB261, were isolated from grass soil sampled in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies showed the two bacteria to be clearly affiliated with the phylum and most closely related to the genus , showing 16S rRNA gene sequence similarities to the type strains of species of the genus of 95.0–96.3 % and sharing 98.5 % similarity between the two strains. The two strains were Gram-stain-positive, aerobic, motile and rod-shaped bacteria. The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H) and MK-9(H). The major fatty acids were iso-C, iso-C, iso-C and summed feature 3 (Cω7 and/or Cω6) for strain PB34 and iso-C, iso-C, iso-C and C for strain PB261. The G+C contents of the genomic DNA of strains PB34 and PB261 were 73.2 mol% and 74.1 mol%, respectively. Thus, based on the evidence of a polyphasic study, it is proposed that strains PB34 and PB261 represent two novel species, for which the names sp. nov. (type strain PB34 = KCTC 19880 = JCM 17785) and sp. nov. (type strain PB261 = KCTC 19881 = JCM 17786) are proposed.

  • Published Online:
Funding
This study was supported by the:
  • Advanced Biomass Research Centre
  • Korean Ministry of Education, Science & Technology
  • CAER
  • Centre for Aquatic Ecosystem Restoration
  • Korean Ministry of Environment
© 2013 IUMS
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2013-07-01
2024-04-19
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Geodermatophilussoli sp. nov. and Geodermatophilusterrae sp. nov., two actinobacteria isolated from grass soil
Int J Syst Evol Microbiol 63, 2625 (2013); https://doi.org/10.1099/ijs.0.048892-0
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