1887

Abstract

A novel strain, designated W-15, was isolated from the gut of a long-horned beetle, , collected in South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the suborder . Strain W-15 was most closely related to RI148-Li105 (97.9 % similarity). Strain W-15 was Gram-stain-positive, rod- and coccus-shaped and non-motile. Growth was observed at 15–37 °C, at pH 4.5–8.5 and in the presence of 0–5.0 % NaCl. The cell-wall peptidoglycan of the strain was A4α (-Lys–-Ser–-Asp). The major menaquinone present in this strain was MK-8 (H) and the major cellular fatty acids were anteiso-C, iso-C, iso-C and anteiso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown lipid, an unknown phospholipid and an unknown phosphoglycolipid. The G+C content of genomic DNA of the strain was 73.8 mol%. On the basis of evidence from our polyphasic taxonomic study, strain W-15 is classified as representing a novel species in the suborder , for which the name sp. nov. is proposed. The type strain of this species is strain W-15 ( = KCTC 19882 = JCM 18090).

Funding
This study was supported by the:
  • Ministry of Education and Science of the Republic of Korea (Award NMM0101232)
  • Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (Award KGM4111241)
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2014-04-01
2024-03-28
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References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  2. Breznak J. A., Costilow R. N. ( 1994 ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  4. Hamada M., Otoguro M., Yamamura H., Tamura T., Suzuki K., Hayakawa M. ( 2010 ). Luteimicrobium subarcticum gen. nov., sp. nov., a new member of the suborder Micrococcineae . . Int J Syst Evol Microbiol 60, 796800. [View Article] [PubMed]
    [Google Scholar]
  5. Hamada M., Yamamura H., Komukai C., Tamura T., Suzuki K., Hayakawa M. ( 2012 ). Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium . . J Antibiot (Tokyo) 65, 427431. [View Article] [PubMed]
    [Google Scholar]
  6. Kim H., Park D. S., Oh H. W., Lee K. H., Chung D. H., Park H. Y., Park H. M., Bae K. S. ( 2012 ). Gryllotalpicola gen. nov., with descriptions of Gryllotalpicola koreensis sp. nov., Gryllotalpicola daejeonensis sp. nov. and Gryllotalpicola kribbensis sp. nov. from the gut of the African mole cricket, Gryllotalpa africana, and reclassification of Curtobacterium ginsengisoli as Gryllotalpicola ginsengisoli comb. nov.. Int J Syst Evol Microbiol 62, 23632370. [View Article] [PubMed]
    [Google Scholar]
  7. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  8. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  9. Sambrook J., Fritsch E. F., Maniatis T. ( 1989 ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  10. Sasser M. ( 2001 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  11. Schumann P. ( 2011 ). Peptidoglycan structure. . Methods Microbiol 38, 101129. [View Article]
    [Google Scholar]
  12. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  13. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
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