1887

Abstract

A Gram-positive-staining, aerobic, endospore-forming bacterium (Ca7) was isolated from a bioreactor showing extensive phosphorus removal. Based on 16S rRNA gene sequence similarity comparisons, strain Ca7 was grouped in the genus , most closely related to JSM 082006 (100 %), V2-BIII-A2 (99.2 %) and Con a/3 (97.7 %). Moderate 16S rRNA gene sequence similarities were found to the type strains of the species and (96.4 %), (95.1 %) and (96.1 %). All these species were grouped into a monophyletic cluster and showed very low sequence similarities (<94 %) to the type species of the genus , . The quinone system of strain Ca7 consists predominantly of menaquinone MK-7. The polar lipid profile exhibited the major compounds diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, minor compounds of an unidentified phospholipid and an aminophospholipid were detected. No glycolipids were found in strain Ca7, which was consistent with the lipid profiles of , , , , , and , but in contrast to . The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid and the polyamine pattern contained predominantly spermidine and spermine. The major fatty acids, which were iso-C, anteiso-C and C, supported the grouping of strain Ca7 in the family . The strain showed DNA–DNA similarities of 48 % (reciprocal 47 %) to DSM 23009, 31 % (reciprocal 36 %) to V2-BIII-A2 and 29 % (reciprocal 39 %) to DSM 15822, respectively. These results clearly demonstrate that strain Ca7 is a representative of a novel species, which can be differentiated from its closest relatives by physiological and biochemical tests. Because of the low sequence similarity of strain Ca7 to , which was shared by , , , , , and , and their unique lipid patterns, we propose that strain Ca7 represents a novel species in a novel genus for which the name gen. nov., sp. nov. is proposed. The type strain is Ca7 ( = CCM 8426 = LMG 27063). In addition we propose the reclassification of , , , , , and as comb. nov., comb. nov., comb. nov comb. nov., comb. nov., comb. nov. and comb. nov., respectively [type strains JSM 082006 ( = DSM 23009 = KCTC 13712), V2-BIII-A2 ( = CCM 4982 = DSM 14730), Con a/3 ( = MTCC 4380 = DSM 15822 = JCM 12167), WPCB074 ( = KCTC 13278 = JCM 16348), ZFHKF-1 ( = JCM 13285 = DSM 17262), YC1 ( = KCTC 13181 = CGMCC 1.6854) and LMG 21881 ( = DSM 15866), respectively].

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2013-08-01
2024-03-29
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References

  1. Aino K., Hirota K., Matsuno T., Morita N., Nodasaka Y., Fujiwara T., Matsuyama H., Yoshimune K., Yumoto I. ( 2008 ). Bacillus polygoni sp. nov., a moderately halophilic, non-motile obligate alkaliphile isolated from indigo balls. . Int J Syst Evol Microbiol 58, 120124. [View Article] [PubMed]
    [Google Scholar]
  2. Albert R. A., Archambault J., Lempa M., Hurst B., Richardson C., Gruenloh S., Duran M., Worliczek H. L., Huber B. E. & other authors ( 2007 ). Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov.. Int J Syst Evol Microbiol 57, 27292737. [View Article] [PubMed]
    [Google Scholar]
  3. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H. J. ( 1996 ). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47, 3952. [View Article]
    [Google Scholar]
  4. Altenburger P., Kämpfer P., Akimov V. N., Lubitz W., Busse H.-J. ( 1997 ). Polyamine distribution in actinomycetes with group B peptidoglycan and species of the genera Brevibacterium, Corynebacterium and Tsukamurella . . Int J Syst Bacteriol 47, 270277. [View Article]
    [Google Scholar]
  5. Arahal D. R., Ventosa A. ( 2002 ). Moderately halophilic and halotolerant species of Bacillus and related genera. . In Applications and Systematics of Bacillus and Relatives, pp. 8399. Edited by Berkeley R. C. W., Heyndrickx M., Logan N., De Vos P. . Oxford:: Blackwell;. [View Article]
    [Google Scholar]
  6. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. ( 1991 ). Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit ribosomal-RNA sequences. . Lett Appl Microbiol 13, 202206. [View Article]
    [Google Scholar]
  7. Bagheri M., Didari M., Amoozegar M. A., Schumann P., Sánchez-Porro C., Mehrshad M., Ventosa A. ( 2012 ). Bacillus iranensis sp. nov., a moderate halophile from a hypersaline lake. . Int J Syst Evol Microbiol 62, 811816. [View Article] [PubMed]
    [Google Scholar]
  8. Baik K. S., Lim C. H., Park S. C., Kim E. M., Rhee M. S., Seong C. N. ( 2010 ). Bacillus rigui sp. nov., isolated from wetland fresh water. . Int J Syst Evol Microbiol 60, 22042209. [View Article] [PubMed]
    [Google Scholar]
  9. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. ( 1978 ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75, 48014805. [View Article] [PubMed]
    [Google Scholar]
  10. Busse J., Auling G. ( 1988 ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . . Syst Appl Microbiol 11, 18. [View Article]
    [Google Scholar]
  11. Carrasco I. J., Márquez M. C., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. ( 2007 ). Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China. . Int J Syst Evol Microbiol 57, 20842088. [View Article] [PubMed]
    [Google Scholar]
  12. Chen Y.-G., Zhang Y.-Q., Wang Y.-X., Liu Z.-X., Klenk H.-P., Xiao H.-D., Tang S.-K., Cui X.-L., Li W. J. ( 2009 ). Bacillus neizhouensis sp. nov., a halophilic marine bacterium isolated from a sea anemone. . Int J Syst Evol Microbiol 59, 30353039. [View Article] [PubMed]
    [Google Scholar]
  13. Chen Y.-G., Zhang L., Zhang Y.-Q., He J.-W., Klenk H.-P., Tang S.-K., Zhang Y.-X., Li W.-J. ( 2011 ). Bacillus nanhaiensis sp. nov., isolated from an oyster. . Int J Syst Evol Microbiol 61, 888893. [View Article] [PubMed]
    [Google Scholar]
  14. Christensen W. B. ( 1946 ). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. . J Bacteriol 52, 461466.[PubMed]
    [Google Scholar]
  15. De Clerck E., Rodríguez-Díaz M., Vanhoutte T., Heyrman J., Logan N. A., De Vos P. ( 2004 ). Anoxybacillus contaminans sp. nov. and Bacillus gelatini sp. nov., isolated from contaminated gelatin batches. . Int J Syst Evol Microbiol 54, 941946. [View Article] [PubMed]
    [Google Scholar]
  16. Felsenstein J. ( 1985 ). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  17. Felsenstein, J. (2005). phylip (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle.
  18. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  19. Gidden J., Denson J., Liyanage R., Ivey D. M., Lay J. O. Jr ( 2009 ). Lipid compositions in Escherichia coli and Bacillus subtilis during growth as determined by MALDI-TOF and TOF/TOF mass spectrometry. . Int J Mass Spectrom 283, 178184. [View Article] [PubMed]
    [Google Scholar]
  20. Hamana K., Akiba T., Uchino F., Matsuzaki S. ( 1989 ). Distribution of spermine in bacilli and lactic acid bacteria. . Can J Microbiol 35, 450455. [View Article] [PubMed]
    [Google Scholar]
  21. Hatayama K., Shoun H., Ueda Y., Nakamura A. ( 2006 ). Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al.1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov.. Int J Syst Evol Microbiol 56, 25452551. [View Article] [PubMed]
    [Google Scholar]
  22. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A. ( 1998 ). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus . . Int J Syst Bacteriol 48, 99106. [View Article]
    [Google Scholar]
  23. Heyndrickx M., Coorevits A., Scheldeman P., Lebbe L., Schumann P., Rodríguez-Diaz M., Forsyth G., Dinsdale A., Heyrman J. & other authors ( 2012 ). Emended descriptions of Bacillus sporothermodurans and Bacillus oleronius with the inclusion of dairy farm isolates of both species. . Int J Syst Evol Microbiol 62, 307314. [View Article] [PubMed]
    [Google Scholar]
  24. Horikoshi K. ( 1999 ). Alkaliphiles: some applications of their products for biotechnology. . Microbiol Mol Biol Rev 63, 735750.[PubMed]
    [Google Scholar]
  25. Iwasaki H., Shimada A., Ito E. ( 1986 ). Comparative studies of lipoteichoic acids from several Bacillus strains. . J Bacteriol 167, 508516.[PubMed]
    [Google Scholar]
  26. Jeon C. O., Lim J.-M., Lee J.-M., Xu L.-H., Jiang C.-L., Kim C.-J. ( 2005 ). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. . Int J Syst Evol Microbiol 55, 18911896. [View Article] [PubMed]
    [Google Scholar]
  27. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of the protein molecules. . In Mammalian protein metabolism, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  28. Kämpfer P. ( 1990 ). Evaluation of the Titertek-Enterobac-Automated System (TTE-AS) for identification of members of the family Enterobacteriaceae . . Zentralbl Bakteriol 273, 164172. [View Article] [PubMed]
    [Google Scholar]
  29. Kämpfer P. ( 1994 ). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. . Syst Appl Microbiol 17, 8698. [View Article] [PubMed]
    [Google Scholar]
  30. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [View Article]
    [Google Scholar]
  31. Kämpfer P., Steiof M., Dott W. ( 1991 ). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21, 227251. [View Article]
    [Google Scholar]
  32. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H.-J., Tindall B. J. ( 2006 ). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56, 781786. [View Article] [PubMed]
    [Google Scholar]
  33. Krulwich T. A., Hicks D. B., Swartz T. H., Ito M. ( 2007 ). Bioenergetics adaptations that support alkaliphily. . In Physiology and Biochemistry of Extremophiles, pp. 311329. Edited by Gerday C., Glansdorff N. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  34. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic acid techniques in bacterial systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  35. Lim J.-M., Jeon C. O., Kim C.-J. ( 2006a ). Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea. . Int J Syst Evol Microbiol 56, 29032908. [View Article] [PubMed]
    [Google Scholar]
  36. Lim J.-M., Jeon C. O., Lee S.-M., Lee J. C., Xu L. H., Jiang C. L., Kim C. J. ( 2006b ). Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China. . Int J Syst Evol Microbiol 56, 373377. [View Article] [PubMed]
    [Google Scholar]
  37. Liu H., Zhou Y., Liu R., Zhang K.-Y., Lai R. ( 2009 ). Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake. . Int J Syst Evol Microbiol 59, 14601464. [View Article] [PubMed]
    [Google Scholar]
  38. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  39. Margesin R., Schinner F. ( 2001 ). Potential of halotolerant and halophilic microorganisms for biotechnology. . Extremophiles 5, 7383. [View Article] [PubMed]
    [Google Scholar]
  40. Munoz R., Yarza P., Ludwig W., Euzéby J., Amann R., Schleifer K. H., Glöckner F. O., Rosselló-Móra R. ( 2011 ). Release LTPs104 of the All-Species Living Tree. . Syst Appl Microbiol 34, 169170. [View Article] [PubMed]
    [Google Scholar]
  41. Nielsen P., Rainey F. A., Outtrup H., Priest F. G., Fritze D. ( 1994 ). Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus . . FEMS Microbiol Lett 117, 6165. [View Article]
    [Google Scholar]
  42. Nogi Y., Takami H., Horikoshi K. ( 2005 ). Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species. . Int J Syst Evol Microbiol 55, 23092315. [View Article] [PubMed]
    [Google Scholar]
  43. Op den Kamp J. A. F., Redai I., van Deenen L. L. ( 1969 ). Phospholipid composition of Bacillus subtilis . . J Bacteriol 99, 298303.[PubMed]
    [Google Scholar]
  44. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . . Nucleic Acids Res 35, 71887196. [View Article] [PubMed]
    [Google Scholar]
  45. Reva O. N., Smirnov V. V., Pettersson B., Priest F. G. ( 2002 ). Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.). . Int J Syst Evol Microbiol 52, 101107. [View Article] [PubMed]
    [Google Scholar]
  46. Romano I., Lama L., Nicolaus B., Gambacorta A., Giordano A. ( 2005 ). Bacillus saliphilus sp. nov., isolated from a mineral pool in Campania, Italy. . Int J Syst Evol Microbiol 55, 159163. [View Article] [PubMed]
    [Google Scholar]
  47. Schleifer K. H. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123156. [View Article]
    [Google Scholar]
  48. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477. [View Article]
    [Google Scholar]
  49. Shivaji S., Suresh K., Chaturvedi P., Dube S., Sengupta S. ( 2005 ). Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India. . Int J Syst Evol Microbiol 55, 11231127. [View Article] [PubMed]
    [Google Scholar]
  50. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [View Article] [PubMed]
    [Google Scholar]
  51. Stolz A., Busse H.-J., Kämpfer P. ( 2007 ). Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57, 572576. [View Article] [PubMed]
    [Google Scholar]
  52. Sumpavapol P., Tongyonk L., Tanasupawat S., Chokesajjawatee N., Luxananil P., Visessanguan W. ( 2010 ). Bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in Thailand. . Int J Syst Evol Microbiol 60, 23642370. [View Article] [PubMed]
    [Google Scholar]
  53. Täubel M., Kämpfer P., Buczolits S., Lubitz W., Busse H.-J. ( 2003 ). Bacillus barbaricus sp. nov., isolated from an experimental wall painting. . Int J Syst Evol Microbiol 53, 725730. [View Article] [PubMed]
    [Google Scholar]
  54. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [View Article]
    [Google Scholar]
  55. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  56. Urdiain M., López-López A., Gonzalo C., Busse H.-J., Langer S., Kämpfer P., Rosselló-Móra R. ( 2008 ). Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense . . Syst Appl Microbiol 31, 339351. [View Article] [PubMed]
    [Google Scholar]
  57. Vaishampayan P., Miyashita M., Ohnishi A., Satomi M., Rooney A., La Duc M. T., Venkateswaran K. ( 2009 ). Description of Rummeliibacillus stabekisii gen. nov., sp. nov. and reclassification of Bacillus pycnus Nakamura et al. 2002 as Rummeliibacillus pycnus comb. nov.. Int J Syst Evol Microbiol 59, 10941099. [View Article] [PubMed]
    [Google Scholar]
  58. Vaz-Moreira I., Figueira V., Lopes A. R., Lobo-da-Cunha A., Spröer C., Schumann P., Nunes O. C., Manaia C. M. ( 2012 ). Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant. . Int J Syst Evol Microbiol 62, 7177. [View Article] [PubMed]
    [Google Scholar]
  59. Ventosa A., Nieto J. J., Oren A. ( 1998 ). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62, 504544.[PubMed]
    [Google Scholar]
  60. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. ( 1999 ). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. Int J Syst Bacteriol 49, 821831. [View Article] [PubMed]
    [Google Scholar]
  61. Wieser M., Worliczek H., Kämpfer P., Busse H.-J. ( 2005 ). Bacillus herbersteinensis sp. nov.. Int J Syst Evol Microbiol 55, 21192123. [View Article] [PubMed]
    [Google Scholar]
  62. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008 ). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31, 241250. [View Article] [PubMed]
    [Google Scholar]
  63. Yoon J.-H., Kang S.-J., Oh T.-K. ( 2007 ). Reclassification of Marinococcus albus Hao et al. 1985 as Salimicrobium album gen. nov., comb. nov. and Bacillus halophilus Ventosa et al. 1990 as Salimicrobium halophilum comb. nov., and description of Salimicrobium luteum sp. nov.. Int J Syst Evol Microbiol 57, 24062411. [View Article] [PubMed]
    [Google Scholar]
  64. Yumoto I. ( 2007 ). Environmental and taxonomic biodiversities of Gram-positive alkaliphiles. . In Physiology and Biochemistry of Extremophiles, pp. 295310. Edited by Gerday C., Glansdorff N. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  65. Zhang T., Fan X., Hanada S., Kamagata Y., Fang H. H. P. ( 2006 ). Bacillus macauensis sp. nov., a long-chain bacterium isolated from a drinking water supply. . Int J Syst Evol Microbiol 56, 349353. [View Article] [PubMed]
    [Google Scholar]
  66. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. ( 1998 ). Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48, 179186. [View Article] [PubMed]
    [Google Scholar]
  67. Zimmermann, J. & Dott, W. (2009a). Recovery of phosphorus from sewage sludge incineration ash by combines bioleaching and bioaccumulation. In International Conference on Nutrient Recovery from Waste Water Streams. Edited by K. Ashley, D. Mavinic, F. Koch. IWA Publishing 2009. ISBN 1843392321.
  68. Zimmermann J., Dott W. ( 2009b ). Sequenced bioleaching and bioaccumulation of phosphorus from sludge combustion. A new way of resource reclaiming. . Adv Mat Res 71–73, 625628. [View Article]
    [Google Scholar]
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