1887

Abstract

Yeasts of the genus were isolated from 651 flower samples collected in Vietnam, using an enrichment medium containing 50 % glucose. Species of the genus were found in 5 % of the samples and 54 strains were isolated. The strains were identified based on D1/D2 LSU rRNA gene sequences as (15 strains), (14 strains), and (2 strains). The remaining 23 strains could not be reliably placed under any known species. Among them, 12 strains isolated from flowers of and were peculiar for the intensive formation of chlamydospores. These strains could be subdivided into pigmented and non-pigmented groups. Both groups were identical in PCR fingerprints generated with primer (GAC) and in D1/D2 and ITS sequences. The yeast was closely related to but differed from the latter by 52 nt (or 10.3 % of divergence) in the D1/D2 sequence and 71 nt (or 16.9 % of divergence) in the ITS sequence. The name sp. nov. is proposed for this novel species. The type strain is TBY 2041.7 = CBS 12757 = NRRL Y-63661. The MycoBank number is MB 803186.

Funding
This study was supported by the:
  • The National Foundation for Science and Technology Development (NAFOSTED) of Vietnam (Award 106.03.137.09)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.049767-0
2013-03-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/1192.html?itemId=/content/journal/ijsem/10.1099/ijs.0.049767-0&mimeType=html&fmt=ahah

References

  1. de Hoog G. S., Smith M. T., Rosa C. A. ( 2011 ). Moniliella Stolk & Dakin (1966). . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 18371846. Edited by Kurtzman C. P., Fell J. W., Boekhout T. . London:: Elsevier;. [View Article]
    [Google Scholar]
  2. Edgar R. C. ( 2004 ). MUSCLE: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32, 17921797. [View Article] [PubMed]
    [Google Scholar]
  3. Esteve-Zarzoso B., Belloch C., Uruburu F., Querol A. ( 1999 ). Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. . Int J Syst Bacteriol 49, 329337. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  5. Inglis G. D., Sigler L., Goettel M. S. ( 1992 ). Trichosporonoides megachiliensis, a new Hyphomycete associated with alfalfa leafcutter bees, with notes on Trichosporonoides and Moniliella . . Mycologia 84, 555570. [View Article]
    [Google Scholar]
  6. Kurtzman C. P., Robnett C. J. ( 1998 ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73, 331371. [View Article] [PubMed]
    [Google Scholar]
  7. Lachance M. A., Starmer W. T., Rosa C. A., Bowles J. M., Barker J. S., Janzen D. H. ( 2001 ). Biogeography of the yeasts of ephemeral flowers and their insects. . FEMS Yeast Res 1, 18.[PubMed] [CrossRef]
    [Google Scholar]
  8. Perko R., DeCock P. ( 2007 ). Erythritol. . In Sweeteners and Sugar Alternatives in Food Technology, pp. 151176. Edited by Mitchell H. . Oxford:: Wiley-Blackwell;.
    [Google Scholar]
  9. Rosa C. A., Jindamorakot S., Limtong S., Nakase T., Lachance M. A., Fidalgo-Jiménez A., Daniel H. M., Pagnocca F. C., Inácio J., Morais P. B. ( 2009 ). Synonymy of the yeast genera Moniliella and Trichosporonoides and proposal of Moniliella fonsecae sp. nov. and five new species combinations. . Int J Syst Evol Microbiol 59, 425429. [View Article] [PubMed]
    [Google Scholar]
  10. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  11. Sawada K., Taki A., Yamakawa T., Seki M. ( 2009 ). Key role for transketolase activity in erythritol production by Trichosporonoides megachiliensis SN-G42. . J Biosci Bioeng 108, 385390. [View Article] [PubMed]
    [Google Scholar]
  12. Sipiczki M. ( 2012 ). Detection of yeast species also occurring in substrates associated with animals and identification of a novel dimorphic species in Verbascum flowers from Georgia. . Antonie van Leeuwenhoek 103, 567576. [PubMed] [CrossRef]
    [Google Scholar]
  13. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  14. Thanh V. N., Hai D. A., Hien D. D., Takashima M., Lachance M. A. ( 2012 ). Moniliella carnis sp. nov. and Moniliella dehoogii sp. nov., two novel species of black yeasts isolated from meat processing environments. . Int J Syst Evol Microbiol 62, 30883094. [View Article] [PubMed]
    [Google Scholar]
  15. Yarrow D. ( 1998 ). Methods for the isolation and identification of yeasts. . In The yeasts, a taxonomic study, , 4th edn., pp. 77100. Edited by Kurtzman C. P., Fell J. W. . Amsterdam:: Elsevier;. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.049767-0
Loading
/content/journal/ijsem/10.1099/ijs.0.049767-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error