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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_1

sp. nov., able to accumulate poly-β-hydroxybutyrate, isolated from a sewage sludge compost–soil microcosm Free

Abstract

A bacterial strain, designated strain LP01, was isolated from a laboratory-scale microcosm packed with a mixture of soil and sewage sludge compost designed to study the evolution of microbial biodiversity over time. The bacterial strain was selected for its potential ability to store polyhydroxyalkanoates (PHAs) as intracellular granules. The cells were aerobic, Gram-stain-negative, non-endospore-forming motile rods. Phylogenetically, the strain was classified within the genus , as its 16S rRNA gene sequence had similarity of 99.2 % with respect to those of DSM 17472 and DSM 17473. DNA–DNA hybridization showed low relatedness of strain LP01 to the type strains of other, phylogenetically related species of the genus . It contained Q-8 as the predominant ubiquinone and summed feature 3 (Cω7 and/or iso-C 2-OH) as the major fatty acid(s). It was found to contain small amounts of the fatty acids C and C 2-OH, a feature that served to distinguish it from its closest phylogenetic relatives within the genus . The DNA G+C content was 66.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data obtained in this study suggest that strain LP01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LP01 ( = CECT 7753 = DSM 26121).

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Funding
This study was supported by the:
  • European Union (Award FP6-032967)
© 2014 IUMS
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2014-01-01
2024-04-26
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Massilia umbonata sp. nov., able to accumulate poly-β-hydroxybutyrate, isolated from a sewage sludge compost–soil microcosm
Int J Syst Evol Microbiol 64, 131 (2014); https://doi.org/10.1099/ijs.0.049874-0
/content/journal/ijsem/10.1099/ijs.0.049874-0
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