1887

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Volume 63, Issue Pt_10

sp. nov., a member of the family isolated from a salt mine, and emended description of the genus Free

Abstract

A thermotolerant, extremely halophilic archaeon, BC12-B1, was isolated from a salt mine in Baicheng county, Xinjiang province, China. Colonies were off-white–grey. The cells stained Gram-negative, were motile and irregularly long-rod-shaped (variation in both width and length) with abundant gas vesicles. The strain was able to grow at 20–55 °C (optimum, 48 °C), at pH 6.0–8.0 (optimum, 7.0–7.3), with 1.8–6.0 M NaCl (optimum, 3.0–3.5 M) and with 0.02–2.2 M Mg (optimum, 0.1–0.2 M). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain BC12-B1 was most closely related to RO5-2 (98.5 %) with less than 95 % sequence similarity to other described species. The genomic DNA G+C content of strain BC12-B1 was 64.0 mol%. The DNA–DNA hybridization value between strain BC12-B1 and RO5-2 was 43.6 %. The major polar lipids of strain BC12-B1 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, four glycolipids and an unknown lipid. Based on phenotypic, chemotaxonomic and genotypic characteristics, strain BC12-B1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BC12-B1 ( = CGMCC 1.12397 = JCM 18758). An emended description of the genus is also provided.

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970002 and 31170001)
© 2013 IUMS
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2013-10-01
2024-04-25
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Halopelagiuslongus sp. nov., a member of the family Halobacteriaceae isolated from a salt mine, and emended description of the genus Halopelagius
Int J Syst Evol Microbiol 63, 3585 (2013); https://doi.org/10.1099/ijs.0.051375-0
/content/journal/ijsem/10.1099/ijs.0.051375-0
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