1887

Abstract

A novel bacterial strain, designated P4, was isolated from Permian groundwater and identified on the basis of its phylogenetic, genotypic, chemotaxonomic and phenotypic characteristics. Cells were aerobic, Gram-stain-negative rods. 16S rRNA gene sequence-based phylogenetic analysis revealed that P4 is affiliated with the family in the phylum , but forms a distinct cluster within this family. The DNA G+C content of strain P4 was 45.2 mol%. The predominant cellular fatty acids were Cω6/Cω7 and iso-C. MK-7 was the main respiratory quinone. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, unidentified phospholipids, an unidentified aminolipid, unidentified glycolipids and unidentified polar lipids. Based on our extensive polyphasic analysis, a novel species in a new genus, gen. nov., sp. nov., is proposed. The type strain of is P4 ( = CICC 10659 = KCTC 32399).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.052662-0
2013-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/4136.html?itemId=/content/journal/ijsem/10.1099/ijs.0.052662-0&mimeType=html&fmt=ahah

References

  1. Chou J.-H., Cho N.-T., Arun A. B., Young C.-C., Chen W.-M. ( 2008 ). Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. . Int J Syst Evol Microbiol 58, 20512055. [View Article] [PubMed]
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [View Article] [PubMed]
    [Google Scholar]
  3. Enticknap J. J., Kelly M., Peraud O., Hill R. T. ( 2006 ). Characterization of a culturable alphaproteobacterial symbiont common to many marine sponges and evidence for vertical transmission via sponge larvae. . Appl Environ Microbiol 72, 37243732. [View Article] [PubMed]
    [Google Scholar]
  4. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  5. Khan S. T., Nakagawa Y., Harayama S. ( 2007 ). Sediminitomix flava gen. nov., sp. nov., of the phylum Bacteroidetes, isolated from marine sediment. . Int J Syst Evol Microbiol 57, 16891693. [View Article] [PubMed]
    [Google Scholar]
  6. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195206. [View Article]
    [Google Scholar]
  7. Nedashkovskaya O. I., Kim S. B., Han S. K., Rhee M. S., Lysenko A. M., Falsen E., Frolova G. M., Mikhailov V. V., Bae K. S. ( 2004a ). Ulvibacter litoralis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the green alga Ulva fenestrata . . Int J Syst Evol Microbiol 54, 119123. [View Article] [PubMed]
    [Google Scholar]
  8. Nedashkovskaya O. I., Vancanneyt M., Van Trappen S., Vandemeulebroecke K., Lysenko A. M., Rohde M., Falsen E., Frolova G. M., Mikhailov V. V., Swings J. ( 2004b ). Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. . Int J Syst Evol Microbiol 54, 17571764. [View Article] [PubMed]
    [Google Scholar]
  9. Nedashkovskaya O. I., Kim S. B., Shin D. S., Beleneva I. A., Mikhailov V. V. ( 2007 ). Fulvivirga kasyanovii gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from seawater in a mussel farm. . Int J Syst Evol Microbiol 57, 10461049. [View Article] [PubMed]
    [Google Scholar]
  10. Seo H.-S., Kwon K. K., Yang S.-H., Lee H.-S., Bae S. S., Lee J.-H., Kim S.-J. ( 2009 ). Marinoscillum gen. nov., a member of the family ‘Flexibacteraceae’, with Marinoscillum pacificum sp. nov. from a marine sponge and Marinoscillum furvescens nom. rev., comb. nov.. Int J Syst Evol Microbiol 59, 12041208. [View Article] [PubMed]
    [Google Scholar]
  11. Srisukchayakul P., Suwanachart C., Sangnoi Y., Kanjana-Opas A., Hosoya S., Yokota A., Arunpairojana V. ( 2007 ). Rapidithrix thailandica gen. nov., sp. nov., a marine gliding bacterium isolated from samples collected from the Andaman sea, along the southern coastline of Thailand. . Int J Syst Evol Microbiol 57, 22752279. [View Article] [PubMed]
    [Google Scholar]
  12. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  13. Wang H., Laughinghouse H. D. IV, Anderson M. A., Chen F., Williams E., Place A. R., Zmora O., Zohar Y., Zheng T., Hill R. T. ( 2012 ). Novel bacterial isolate from Permian groundwater, capable of aggregating potential biofuel-producing microalga Nannochloropsis oceanica IMET1. . Appl Environ Microbiol 78, 14451453. [View Article] [PubMed]
    [Google Scholar]
  14. Yi H., Chun J. ( 2004 ). Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 54, 157162. [View Article] [PubMed]
    [Google Scholar]
  15. Yi H., Chang Y.-H., Oh H. W., Bae K. S., Chun J. ( 2003 ). Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments. . Int J Syst Evol Microbiol 53, 10131018. [View Article] [PubMed]
    [Google Scholar]
  16. Yoon J., Ishikawa S., Kasai H., Yokota A. ( 2007 ). Perexilibacter aurantiacus gen. nov., sp. nov., a novel member of the family ‘Flammeovirgaceae’ isolated from sediment. . Int J Syst Evol Microbiol 57, 964968. [View Article] [PubMed]
    [Google Scholar]
  17. Yoon J., Matsuo Y., Kasai H., Yokota A. ( 2008 ). Limibacter armeniacum gen. nov., sp. nov., a novel representative of the family ‘Flammeovirgaceae’ isolated from marine sediment. . Int J Syst Evol Microbiol 58, 982986. [View Article] [PubMed]
    [Google Scholar]
  18. Yoon J., Adachi K., Park S., Kasai H., Yokota A. ( 2011 ). Aureibacter tunicatorum gen. nov., sp. nov., a marine bacterium isolated from a coral reef sea squirt, and description of Flammeovirgaceae fam. nov.. Int J Syst Evol Microbiol 61, 23422347. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.052662-0
Loading
/content/journal/ijsem/10.1099/ijs.0.052662-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error