RT Journal Article SR Electronic(1) A1 den Bakker, Henk C. A1 Warchocki, Steven A1 Wright, Emily M. A1 Allred, Adam F. A1 Ahlstrom, Christina A1 Manuel, Clyde S. A1 Stasiewicz, Matthew J. A1 Burrell, Angela A1 Roof, Sherry A1 Strawn, Laura K. A1 Fortes, Esther A1 Nightingale, Kendra K. A1 Kephart, Daniel A1 Wiedmann, MartinYR 2014 T1 Listeria floridensis sp. nov., Listeria aquatica sp. nov., Listeria cornellensis sp. nov., Listeria riparia sp. nov. and Listeria grandensis sp. nov., from agricultural and natural environments JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_6 SP 1882 OP 1889 DO https://doi.org/10.1099/ijs.0.052720-0 PB Microbiology Society, SN 1466-5034, AB Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average blast nucleotide identity (ANIb) of less than 85 % to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria ; (i) a clade representing Listeria monocytogenes , L. marthii , L. innocua , L. welshimeri , L. seeligeri and L. ivanovii , which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188T = DSM 26686T = LMG 28120T = BEI NR-42633T) and Listeria floridensis sp. nov. (type strain FSL S10-1187T = DSM 26687T = LMG 28121T = BEI NR-42632T), (iii) a clade consisting of Listeria rocourtiae , L. weihenstephanensis and three novel species, Listeria cornellensis sp. nov. (type strain TTU A1-0210T = FSL F6-0969T = DSM 26689T = LMG 28123T = BEI NR-42630T), Listeria grandensis sp. nov. (type strain TTU A1-0212T = FSL F6-0971T = DSM 26688T = LMG 28122T = BEI NR-42631T) and Listeria riparia sp. nov. (type strain FSL S10-1204T = DSM 26685T = LMG 28119T = BEI NR- 42634T) and (iv) a clade containing Listeria grayi . Genomic and phenotypic data suggest that the novel species are non-pathogenic., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.052720-0