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Abstract

Four Gram-staining-positive, catalase-negative, coccoid isolates, designated NTUH_1465, NTUH_2196, NTUH_4957 and NTUH_5572, were isolated from human specimens. The four isolates displayed more than 99.6 % 16S rRNA gene sequence similarity with ATCC 10379, and 96.7 to 98.6 % similarity with ATCC 700632, ATCC 27824 or CCUG 42726. However, phylogenetic analysis of concatenated sequences of three housekeeping genes, , and , suggested that the four isolates were distinct from ATCC 10379 and other species. Isolates NTUH_2196, NTUH_4957 and NTUH_5572 clustered together and formed a stable monophyletic clade. DNA–DNA hybridization values among strains NTUH_1465 and NTUH_5572 and their phylogenetically related neighbours were all lower than 49 %. The four isolates could be distinguished from and other species by phenotypic characteristics. Based on the phylogenetic and phenotypic results, two novel species sp. nov. (type strain NTUH_1465 = BCRC 80365 = JCM 18067) and sp. nov. (type strain NTUH_5572 = BCRC 80366 = JCM 18066) are proposed.

Funding
This study was supported by the:
  • National Science Council of Taiwan (Award NSC 97-2320-B-002-023-MY3)
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2014-06-01
2024-03-19
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References

  1. Anil M., Ozkalay N., Helvaci M., Agus N., Guler O., Dikerler A., Kanar B. ( 2007 ). Meningitis due to Gemella haemolysans in a pediatric case. . J Clin Microbiol 45, 23372339. [View Article] [PubMed]
    [Google Scholar]
  2. Berger U. ( 1961 ). A proposed new genus of Gram-negative cocci: Gemella . . Int Bull Bacteriol Nomencl Taxon 11, 1719. [View Article]
    [Google Scholar]
  3. Brooks J. B., Kellogg D. S., Thacker L., Turner E. M. ( 1971 ). Analysis by gas chromatography of fatty acids found in whole cultural extracts of Neisseria species. . Can J Microbiol 17, 531543. [View Article] [PubMed]
    [Google Scholar]
  4. Brouqui P., Raoult D. ( 2001 ). Endocarditis due to rare and fastidious bacteria. . Clin Microbiol Rev 14, 177207. [View Article] [PubMed]
    [Google Scholar]
  5. Cerdá Zolezzi P., Laplana L. M., Calvo C. R., Cepero P. G., Erazo M. C., Gómez-Lus R. ( 2004 ). Molecular basis of resistance to macrolides and other antibiotics in commensal viridans group streptococci and Gemella spp. and transfer of resistance genes to Streptococcus pneumoniae . . Antimicrob Agents Chemother 48, 34623467. [View Article] [PubMed]
    [Google Scholar]
  6. CLSI ( 2010 ). Performance Standards for Antimicrobial Susceptibility Testing; 20th Informational Supplement M100-S20. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  7. Collins M. D. ( 2006 ). The Genus Gemella . . In The Prokaryotes, , 3rd edn., pp. 511518. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  8. Collins M. D., Hutson R. A., Falsen E., Sjöden B., Facklam R. R. ( 1998a ). Description of Gemella sanguinis sp. nov., isolated from human clinical specimens. . J Clin Microbiol 36, 30903093.[PubMed]
    [Google Scholar]
  9. Collins M. D., Hutson R. A., Falsen E., Sjöden B., Facklam R. R. ( 1998b ). Gemella bergeriae sp. nov., isolated from human clinical specimens. . J Clin Microbiol 36, 12901293.[PubMed]
    [Google Scholar]
  10. Collins M. D., Rodriguez Jovita M., Foster G., Sjödén B., Falsen E. ( 1999 ). Characterization of a Gemella-like organism from the oral cavity of a dog: description of Gemella palaticanis sp. nov.. Int J Syst Bacteriol 49, 15231526. [View Article] [PubMed]
    [Google Scholar]
  11. Durack D. T., Kaplan E. L., Bisno A. L. ( 1983 ). Apparent failures of endocarditis prophylaxis. Analysis of 52 cases submitted to a national registry. . JAMA 250, 23182322. [View Article] [PubMed]
    [Google Scholar]
  12. Gevers D., Cohan F. M., Lawrence J. G., Spratt B. G., Coenye T., Feil E. J., Stackebrandt E., Van de Peer Y., Vandamme P. & other authors ( 2005 ). Opinion: re-evaluating prokaryotic species. . Nat Rev Microbiol 3, 733739. [View Article] [PubMed]
    [Google Scholar]
  13. Hanage W. P., Fraser C., Spratt B. G. ( 2006 ). Sequences, sequence clusters and bacterial species. . Philos Trans R Soc Lond B Biol Sci 361, 19171927. [View Article] [PubMed]
    [Google Scholar]
  14. Hoyles L., Foster G., Falsen E., Collins M. D. ( 2000 ). Characterization of a Gemella-like organism isolated from an abscess of a rabbit: description of Gemella cunicula sp. nov.. Int J Syst Evol Microbiol 50, 20372041. [View Article] [PubMed]
    [Google Scholar]
  15. Hung W.-C., Tseng S.-P., Chen H.-J., Tsai J.-C., Chang C.-H., Lee T.-F., Hsueh P.-R., Teng L.-J. ( 2010 ). Use of groESL as a target for identification of Abiotrophia, Granulicatella, and Gemella species. . J Clin Microbiol 48, 35323538. [View Article] [PubMed]
    [Google Scholar]
  16. Jean W. D., Leu T.-Y., Lee C.-Y., Chu T.-J., Lin S. Y., Shieh W. Y. ( 2009 ). Pseudidiomarina marina sp. nov. and Pseudidiomarina tainanensis sp. nov. and reclassification of Idiomarina homiensis and Idiomarina salinarum as Pseudidiomarina homiensis comb. nov. and Pseudidiomarina salinarum comb. nov., respectively. . Int J Syst Evol Microbiol 59, 5359. [View Article] [PubMed]
    [Google Scholar]
  17. Kilpper-Bälz R., Schleifer K. H. ( 1988 ). Transfer of Streptococcus morbillorum to the genus Gemella as Gemella morbillorum comb. nov.. Int J Syst Bacteriol 38, 442443. [View Article]
    [Google Scholar]
  18. Konstantinidis K. T., Ramette A., Tiedje J. M. ( 2006 ). Toward a more robust assessment of intraspecies diversity, using fewer genetic markers. . Appl Environ Microbiol 72, 72867293. [View Article] [PubMed]
    [Google Scholar]
  19. La Scola B., Raoult D. ( 1998 ). Molecular identification of Gemella species from three patients with endocarditis. . J Clin Microbiol 36, 866871.[PubMed]
    [Google Scholar]
  20. Malik I., Ghosh S., Nutt C., Macdonald A., Bal A. M., Collier A. ( 2010 ). Gemella haemolysans bacteraemia in a patient with solitary liver abscess. . J Microbiol Immunol Infect 43, 438441. [View Article] [PubMed]
    [Google Scholar]
  21. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A., Kämpfer P., Maiden M. C., Nesme X., Rosselló-Mora R., Swings J. & other authors ( 2002 ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52, 10431047. [View Article] [PubMed]
    [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  23. Thjötta T., Böe J. ( 1938 ). Neisseria haemolysans. A haemolytic species of Neisseria Trevisan. . Acta Pathol Microbiol Scand Suppl 37, 727731.
    [Google Scholar]
  24. Thompson C. C., Thompson F. L., Vandemeulebroecke K., Hoste B., Dawyndt P., Swings J. ( 2004 ). Use of recA as an alternative phylogenetic marker in the family Vibrionaceae . . Int J Syst Evol Microbiol 54, 919924. [View Article] [PubMed]
    [Google Scholar]
  25. Thompson F. L., Gevers D., Thompson C. C., Dawyndt P., Naser S., Hoste B., Munn C. B., Swings J. ( 2005 ). Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. . Appl Environ Microbiol 71, 51075115. [View Article] [PubMed]
    [Google Scholar]
  26. Ulger-Toprak N., Summanen P. H., Liu C., Rowlinson M. C., Finegold S. M. ( 2010 ). Gemella asaccharolytica sp. nov., isolated from human clinical specimens. . Int J Syst Evol Microbiol 60, 10231026. [View Article] [PubMed]
    [Google Scholar]
  27. Vossen M. G., Gattringer K. B., Khalifeh N., Koreny M., Spertini V., Mallouhi A., Willeit M., Volc-Platzer B., Asboth F. & other authors ( 2012 ). Gemella morbillorum bacteremia after anti-tumor necrosis factor alpha as acne inversa therapy. . J Clin Microbiol 50, 11091112. [View Article] [PubMed]
    [Google Scholar]
  28. Woo P. C., To A. P., Lau S. K., Fung A. M., Yuen K. Y. ( 2004 ). Phenotypic and molecular characterization of erythromycin resistance in four isolates of Streptococcus-like gram-positive cocci causing bacteremia. . J Clin Microbiol 42, 33033305. [View Article] [PubMed]
    [Google Scholar]
  29. Yamakawa T., Ueta N. ( 1964 ). Gaschromatographic studies of microbial components. I. Carbohydrate and fatty ccid constitution of Neisseria . . Jpn J Exp Med 34, 361374.[PubMed]
    [Google Scholar]
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