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Volume 63, Issue Pt_11

sp. nov., from a marine solar saltern and an inland salt lake of China Free

Abstract

Two halophilic archaeal strains, YC20 and XD15, were isolated from a marine solar saltern and an inland salt lake in China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They were neutrophilic, requiring at least 100 g NaCl l and 0.5–95 g MgCl l for growth at the optimum growth temperature of 37 °C. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified glycolipids were also detected. The 16S rRNA gene sequences of the two strains were 99.5 % identical and showed 94.0–95.9 % similarity to the most closely related members of the genus of the family . The gene sequence similarity between strains YC20 and XD15 was 98.2 % and these sequences showed 89.6–92.8 % similarity to those of the most closely related members of the genus . The DNA G+C contents of strains YC20 and XD15 were 65.8 mol% and 65.4 mol%, respectively. The DNA–DNA hybridization value between strain YC20 and strain XD15 was 92 %, and the two strains showed low DNA–DNA relatedness to members of the genus . The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains YC20 and XD15 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YC20 ( = CGMCC 1.12120 = JCM 18361) and the other strain is XD15 ( = CGMCC 1.12236 = JCM 18648).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970006)
  • State Key Laboratory of Microbial Resources (Award SKLMR-20100604)
  • Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin (Award BRZD1206)
  • Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
© 2013 IUMS
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2013-11-01
2024-04-24
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References

  1. Castillo A. M., Gutiérrez M. C., Kamekura M., Ma Y. H., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. ( 2006a ). Halovivax asiaticus gen. nov., sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. . Int J Syst Evol Microbiol 56, 765–770. [View Article] [PubMed]
    [Google Scholar]
  2. Castillo A. M., Gutiérrez M. C., Kamekura M., Xue Y. F., Ma Y. H., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. ( 2006b ). Halostagnicola larsenii gen. nov., sp. nov., an extremely halophilic archaeon from a saline lake in Inner Mongolia, China. . Int J Syst Evol Microbiol 56, 1519–1524. [View Article] [PubMed]
    [Google Scholar]
  3. Cui H. L., Lin Z. Y., Dong Y., Zhou P. J., Liu S. J. ( 2007 ). Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. . Int J Syst Evol Microbiol 57, 2204–2206. [View Article] [PubMed]
    [Google Scholar]
  4. Cui H.-L., Zhou P.-J., Oren A., Liu S.-J. ( 2009 ). Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium . . Extremophiles 13, 31–37. [View Article] [PubMed]
    [Google Scholar]
  5. Cui H.-L., Gao X., Sun F.-F., Dong Y., Xu X.-W., Zhou Y.-G., Liu H.-C., Oren A., Zhou P.-J. ( 2010a ). Halogranum rubrum gen. nov., sp. nov., a halophilic archaeon isolated from a marine solar saltern. . Int J Syst Evol Microbiol 60, 1366–1371. [View Article] [PubMed]
    [Google Scholar]
  6. <1?tic r?> Cui H.-L., Gao X., Yang X., Xu X.-W. ( 2010b ). Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. . Extremophiles 14, 493–499. [View Article] [PubMed]
    [Google Scholar]
  7. Cui H.-L., Li X.-Y., Gao X., Xu X.-W., Zhou Y.-G., Liu H.-C., Oren A., Zhou P.-J. ( 2010c ). Halopelagius inordinatus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. . Int J Syst Evol Microbiol 60, 2089–2093. [View Article] [PubMed]
    [Google Scholar]
  8. Cui H.-L., Gao X., Yang X., Xu X.-W. ( 2011a ). Halolamina pelagica gen. nov., sp. nov., a new member of the family Halobacteriaceae . . Int J Syst Evol Microbiol 61, 1617–1621. [View Article] [PubMed]
    [Google Scholar]
  9. Cui H.-L., Yang X., Gao X., Xu X.-W. ( 2011b ). Halobellus clavatus gen. nov., sp. nov. and Halorientalis regularis gen. nov., sp. nov., two new members of the family Halobacteriaceae . . Int J Syst Evol Microbiol 61, 2682–2689. [View Article] [PubMed]
    [Google Scholar]
  10. Cui H.-L., Yang X., Mou Y. Z. ( 2011c ). Salinarchaeum laminariae gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from salted brown alga Laminaria . . Extremophiles 15, 625–631. [View Article] [PubMed]
    [Google Scholar]
  11. Cui H.-L., Mou Y. Z., Yang X., Zhou Y. G., Liu H. C., Zhou P. J. ( 2012a ). Halorubellus salinus gen. nov., sp. nov. and Halorubellus litoreus sp. nov., novel halophilic archaea isolated from a marine solar saltern. . Syst Appl Microbiol 35, 30–34. [View Article] [PubMed]
    [Google Scholar]
  12. Cui H.-L., Yang X., Zhou Y. G., Liu H. C., Zhou P. J., Dyall-Smith M. L. ( 2012b ). Halobellus limi sp. nov. and Halobellus salinus sp. nov., isolated from two marine solar salterns. . Int J Syst Evol Microbiol 62, 1307–1313. [View Article] [PubMed]
    [Google Scholar]
  13. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  14. Dussault H. P. ( 1955 ). An improved technique for staining red halophilic bacteria. . J Bacteriol 70, 484–485.[PubMed]
    [Google Scholar]
  15. Gonzalez C., Gutierrez C., Ramirez C. ( 1978 ). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. . Can J Microbiol 24, 710–715. [View Article] [PubMed]
    [Google Scholar]
  16. Gutiérrez C., González C. ( 1972 ). Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. . Appl Microbiol 24, 516–517.[PubMed]
    [Google Scholar]
  17. Gutiérrez M. C., Castillo A. M., Kamekura M., Xue Y. F., Ma Y. H., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. ( 2007 ). Halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from saline Lake Shangmatala in Inner Mongolia, China. . Int J Syst Evol Microbiol 57, 1402–1407. [View Article] [PubMed]
    [Google Scholar]
  18. Huß V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  19. Itoh T., Yamaguchi T., Zhou P. J., Takashina T. ( 2005 ). Natronolimnobius baerhuensis gen. nov., sp. nov. and Natronolimnobius innermongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia, China. . Extremophiles 9, 111–116. [View Article] [PubMed]
    [Google Scholar]
  20. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109–118. [View Article] [PubMed]
    [Google Scholar]
  21. McDade J. J., Weaver R. H. ( 1959 ). Rapid methods for the detection of gelatin hydrolysis. . J Bacteriol 77, 60–64.[PubMed]
    [Google Scholar]
  22. Minegishi H., Kamekura M., Itoh T., Echigo A., Usami R., Hashimoto T. ( 2010 ). Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B′ (rpoB′) gene. . Int J Syst Evol Microbiol 60, 2398–2408. [View Article] [PubMed]
    [Google Scholar]
  23. Mou Y. Z., Qiu X. X., Zhao M. L., Cui H. L., Oh D., Dyall-Smith M. L. ( 2012 ). Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively. . Extremophiles 16, 895–901. [View Article] [PubMed]
    [Google Scholar]
  24. Oren A., Ventosa A., Grant W. D. ( 1997 ). Proposed minimal standards for description of new taxa in the order Halobacteriales . . Int J Syst Bacteriol 47, 233–238. [View Article]
    [Google Scholar]
  25. Owen R. J., Pitcher D. ( 1985 ). Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. . In Chemical Methods in Bacterial Systematics, pp. 67–93. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  26. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  28. Xu Y., Zhou P. J., Tian X. Y. ( 1999 ). Characterization of two novel haloalkaliphilic archaea Natronorubrum bangense gen. nov., sp. nov. and Natronorubrum tibetense gen. nov., sp. nov.. Int J Syst Bacteriol 49, 261–266. [View Article] [PubMed]
    [Google Scholar]
  29. Zheng M. P., Tang J. Y., Liu J. Y., Zhang F. S. ( 1993 ). Chinese saline lakes. . Hydrobiologia 267, 23–36. [View Article]
    [Google Scholar]
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Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China
Int J Syst Evol Microbiol 63, 3975 (2013); https://doi.org/10.1099/ijs.0.053066-0
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