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Volume 64, Issue Pt_1

sp. nov., a halophilic bacterium from a hypersaline lake Free

Abstract

A Gram-positive, endospore-forming, rod-shaped bacterium, designated isolate J4, was isolated from a neutral saline lake sample from Xinjiang Uyghur Autonomous Region, China, and subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain J4 is most closely related to 25-7 (with 96.7 % similarity), 8-2 (96.5 %), TW25 (96.4 %), CVS-14 (96.4 %) and MB-9 (96.2 %). Chemotaxonomic analysis showed menaquinone-7 (MK-7) to be the major isoprenoid quinone of strain J4; diphosphatidylglycerol and phosphatidylglycerol were the major cellular polar lipids and the cell wall contained -diaminopimelic acid as the diagnostic diamino acid. The major cellular fatty acids were iso-C and anteiso-C. The genomic DNA G+C content of strain J4 was determined to be 36.2 mol%. Strain J4 was positive for catalase activity and negative for oxidase activity. Strain J4 was observed to grow at 25–50 °C (optimal 35–42 °C), pH 6.5–8.0 (optimal 7.0–7.5) and in media containing 1–21 % (w/v) NaCl (optimal 9–12 %). Based on these data, strain J4 represents a novel species of the genus and the name sp. nov. is proposed. The type strain is J4 ( = CGMCC 1.12331 = JCM 18732).

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Funding
This study was supported by the:
  • Ministry of Science and Technology of China (Award 2011ZX11102-011-11, 2012CB721000 and 2013CB734000)
  • National Natural Science Foundation of China (Award 31000004, 31170095, 31100075 and 81261120389)
  • C. A. S. Pillar Program (Award XDA04074000)
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2014-01-01
2024-04-18
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Salinibacillus xinjiangensis sp. nov., a halophilic bacterium from a hypersaline lake
Int J Syst Evol Microbiol 64, 27 (2014); https://doi.org/10.1099/ijs.0.053306-0
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