1887

Abstract

Two strains (NYNU 121010 and NYNU 121032) of a novel basidiomycetous yeast species belonging to the genus were isolated from insect frass collected from trunks of a pagoda tree ( L.) in Yantai, Shandong province, east China. The sequence analyses of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region indicated that the closest relatives were FIRDI 007, CBS 7429 and sp. S6A. The D1/D2 sequences of the novel strains differed by 12 nt substitutions (2 %) from the type strain of . , and by 13 nt substitutions (2.2 %) from the type strain of . and from sp. S6A. The novel strains differed from closely related species by more than 4.6 % substitutions in the ITS region. The novel strains can also be distinguished from . and . on the basis of a number of morphological and physiological characteristics and represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is NYNU 121010 ( = CICC 32998 = CBS 12813). The Mycobank deposit number is MB 804119.

Funding
This study was supported by the:
  • Research Planning Project of Basic and Advanced Technology of Henan Province, China (Award 122300410032)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053686-0
2013-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3501.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053686-0&mimeType=html&fmt=ahah

References

  1. Begerow D., Bauer R., Oberwinkler F. ( 2001 ). Muribasidiospora: Microstromatales or Exobasidiales? . Mycol Res 105, 798810. [View Article]
    [Google Scholar]
  2. Begerow D., Stoll M., Bauer R. ( 2006 ). A phylogenetic hypothesis of Ustilaginomycotina based on multiple gene analyses and morphological data. . Mycologia 98, 906916. [View Article] [PubMed]
    [Google Scholar]
  3. de Beer Z. W., Begerow D., Bauer R., Pegg G. S., Crous P. W., Wingfield M. J. ( 2006 ). Phylogeny of the Quambalariaceae fam. nov., including important Eucalyptus pathogens in South Africa and Australia. . Stud Mycol 55, 289298. [View Article] [PubMed]
    [Google Scholar]
  4. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. ( 2000 ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. . Int J Syst Evol Microbiol 50, 13511371. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  6. Golubev V. I., Kulakovskaia T. V., Kulakovskaia E. V., Golubev N. V. ( 2004 ). [ The fungicidal activity of an extracellular glycolipid from Sympodiomycopsis paphiopedili Sugiyama et al.. ]. Mikrobiologiia 73, 841845 (in Russian).[PubMed]
    [Google Scholar]
  7. Hamamoto M., Nagahama T., Tamura M. ( 2002 ). Systematic study of basidiomycetous yeasts - evaluation of the ITS regions of rDNA to delimit species of the genus Rhodosporidium . . FEMS Yeast Res 2, 409413.[PubMed]
    [Google Scholar]
  8. Jindamorakot S., Am-In S., Thuy T. T., Duy N. D., Kawasaki H., Potacharoen W., Limtong S., Tanticharoen M., Nakase T. ( 2004 ). Candida easanensis sp. nov., Candida pattaniensis sp. nov. and Candida nakhonratchasimensis sp. nov., three new species of yeasts isolated from insect frass in Thailand. . J Gen Appl Microbiol 50, 261269. [View Article] [PubMed]
    [Google Scholar]
  9. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  10. Kulakovskaya T. V., Shashkov A. S., Kulakovskaya E. V., Golubev W. I. ( 2004 ). Characterization of an antifungal glycolipid secreted by the yeast Sympodiomycopsis paphiopedili . . FEMS Yeast Res 5, 247252. [View Article] [PubMed]
    [Google Scholar]
  11. Kurtzman C. P., Robnett C. J. ( 1998 ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73, 331371. [View Article] [PubMed]
    [Google Scholar]
  12. Mahdi L. E., Statzell-Tallman A., Fell J. W., Brown M. V., Donachie S. P. ( 2008 ). Sympodiomycopsis lanaiensis sp. nov., a basidiomycetous yeast (Ustilaginomycotina: Microstromatales) from marine driftwood in Hawai’i. . FEMS Yeast Res 8, 13571363. [View Article] [PubMed]
    [Google Scholar]
  13. Nakase T., Suzuki M. ( 1986 ). Bullera megalospora, a new species of yeast forming larger ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. . J Gen Appl Microbiol 32, 225240. [View Article]
    [Google Scholar]
  14. Ramos J. P., Valente P., De Souza R. A., Rosa C. A., Leoncini O. ( 2001 ). Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species. . Lett Åppl Microbiol 33, 206210. [View Article] [PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  16. Sipiczki M., Kajdacsi E. ( 2009 ). Jaminaea angkorensis gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales. . Int J Syst Evol Microbiol 59, 914920. [View Article] [PubMed]
    [Google Scholar]
  17. Sugiyama J., Tokuoka K., Suh S.-O., Hirata A., Komagata K. ( 1991 ). Sympodiomycopsis: a new yeast-like anamorph genus with basidiomycetous nature from orchid nectar. . Antonie van Leeuwenhoek 59, 95108. [View Article] [PubMed]
    [Google Scholar]
  18. Thanh V. N., Hai D. A., Lachance M. A. ( 2003 ). Issatchenkia hanoiensis, a new yeast species isolated from frass of the litchi fruit borer Conopomorpha cramerella Snellen. . FEMS Yeast Res 4, 113117. [View Article] [PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  20. Wei Y. H., Liou G. Y., Liu H. Y., Lee F. L. ( 2011 ). Sympodiomycopsis kandeliae sp. nov., a basidiomycetous anamorphic fungus from mangroves, and reclassification of Sympodiomycopsis lanaiensis as Jaminaea lanaiensis comb. nov.. Int J Syst Evol Microbiol 61, 469473. [View Article] [PubMed]
    [Google Scholar]
  21. White T. J., Bruns T., Lee S., Taylor J. ( 1990 ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. . In PCR Protocols: a Guide to Methods and Applications, pp. 315322. Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. . San Diego, CA:: Academic Press;.
    [Google Scholar]
  22. Yamada Y., Kondo K. ( 1973 ). Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium . . J Gen Appl Microbiol 19, 5977. [View Article]
    [Google Scholar]
  23. Yarrow D. ( 1998 ). Methods for the isolation, maintenance and identification of yeasts. . In The Yeasts, a Taxonomic Study, , 4th edn., pp. 77100. Edited by Kurtzman C. P., Fell J. W. . Amsterdam:: Elsevier;. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.053686-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053686-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error