@article{mbs:/content/journal/ijsem/10.1099/ijs.0.053736-0, author = "Felföldi, Tamás and Vengring, Anita and Márialigeti, Károly and András, Judit and Schumann, Peter and Tóth, Erika M.", title = "Hephaestia caeni gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from activated sludge", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_3", pages = "738-744", doi = "https://doi.org/10.1099/ijs.0.053736-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.053736-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-staining-negative, rod-shaped and motile bacterium, designated strain ERB1-3T, was isolated from a laboratory-scale activated sludge system treating coke plant effluent using thiocyanate-supplemented growth medium. Strain ERB1-3T was oxidase-positive and weakly catalase-positive. The predominant fatty acids were C18 : 1ω7c (35.6 %) and C17 : 1ω6c (29.2 %), and the major respiratory quinone was Q-10. Polar lipids were dominated by sphingoglycolipid and phosphatidylglycerol. Major polyamines were spermidine and sym-homospermidine. The G+C content of the genomic DNA of strain ERB1-3T was 66.4 mol%. Based on the 16S rRNA gene, strain ERB1-3T exhibited the highest sequence similarity values to Sphingomonas sanxanigenens DSM 19645T (96.1 %), Sphingobium scionense DSM 19371T (95.1 %) and Stakelama pacifica LMG 24686T (94.8 %) within the family Sphingomonadaceae . The novel isolate had some unique chemotaxonomic features that differentiated it from these closely related strains, contained much more C17 : 1ω6c, C15 : 0 2-OH, C17 : 0 and C17 : 1ω8c fatty acids and possessed diphosphatidylglycerol only in trace amounts. On the basis of the phenotypic, chemotaxonomic and molecular data, strain ERB1-3T is considered to represent a novel genus and species, for which the name Hephaestia caeni gen. nov., sp. nov. is proposed. The type strain is ERB1-3T ( = DSM 25527T = NCAIM B 02511T).", }