@article{mbs:/content/journal/ijsem/10.1099/ijs.0.054015-0, author = "Cao, Junwei and Lai, Qiliang and Liu, Yang and Li, Guizhen and Shao, Zongze", title = "Ottowia beijingensis sp. nov., isolated from coking wastewater activated sludge, and emended description of the genus Ottowia", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_3", pages = "963-967", doi = "https://doi.org/10.1099/ijs.0.054015-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.054015-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A taxonomic study was carried out on strain GCS-AN-3T, which was isolated from a phenol-degrading consortium enriched from coking wastewater activated sludge of Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AN-3T were Gram-stain-negative, short rods, and oxidase-/catalase-positive. Growth was observed at salinities from 0 to 2.5 % and at temperatures from 10 to 37 °C. 16S rRNA gene sequence analysis showed that strain GCS-AN-3T was most closely related to Ottowia pentelensis DSM 21699T (96.2 %). The principal fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 0, summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) and cyclo C17 : 0. The major respiratory quinone was Q-8. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA was 67.6 mol%. Thiosulfate could be utilized as co-substrate for aerobic growth and was oxidized to sulfate. On the basis of phenotypic, chemotaxonomic and molecular data, strain GCS-AN-3T is considered to represent a novel species of the genus Ottowia , for which the name Ottowia beijingensis sp. nov. is proposed (type strain GCS-AN-3T = LMG 27179T = CGMCC 1.12324T = MCCC 1A01410T). An emended description of the genus Ottowia is also proposed.", }