RT Journal Article SR Electronic(1) A1 Cao, Junwei A1 Lai, Qiliang A1 Liu, Yang A1 Li, Guizhen A1 Shao, ZongzeYR 2014 T1 Ottowia beijingensis sp. nov., isolated from coking wastewater activated sludge, and emended description of the genus Ottowia JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_3 SP 963 OP 967 DO https://doi.org/10.1099/ijs.0.054015-0 PB Microbiology Society, SN 1466-5034, AB A taxonomic study was carried out on strain GCS-AN-3T, which was isolated from a phenol-degrading consortium enriched from coking wastewater activated sludge of Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AN-3T were Gram-stain-negative, short rods, and oxidase-/catalase-positive. Growth was observed at salinities from 0 to 2.5 % and at temperatures from 10 to 37 °C. 16S rRNA gene sequence analysis showed that strain GCS-AN-3T was most closely related to Ottowia pentelensis DSM 21699T (96.2 %). The principal fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 0, summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) and cyclo C17 : 0. The major respiratory quinone was Q-8. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA was 67.6 mol%. Thiosulfate could be utilized as co-substrate for aerobic growth and was oxidized to sulfate. On the basis of phenotypic, chemotaxonomic and molecular data, strain GCS-AN-3T is considered to represent a novel species of the genus Ottowia , for which the name Ottowia beijingensis sp. nov. is proposed (type strain GCS-AN-3T = LMG 27179T = CGMCC 1.12324T = MCCC 1A01410T). An emended description of the genus Ottowia is also proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.054015-0