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Volume 64, Issue Pt_4

sp. nov., isolated from sediment of a eutrophic reservoir Free

Abstract

Strain CH15-11, isolated from a sediment sample taken from Daechung Reservoir, South Korea, during the late-blooming period of cyanobacteria, was found to be a Gram-stain-negative, non-motile, non-spore-forming, rod-shaped and aerobic bacterium. Strain CH15-11 grew optimally at pH 7 and 28–30 °C. According to a phylogenetic tree based on 16S rRNA gene sequences, strain CH15-11 belonged to the genus and clustered with Dae 20, with which it shared the highest 16S rRNA gene sequence similarity (97.6 %). Chemotaxonomic analysis showed that strain CH15-11 had characteristics typical of members of the genus , such as the presence of sphingoglycolipid, ubiquinone Q-10 and -homospermidine. Plus, strain CH15-11 included summed feature 8 (Cω7 and/or Cω6) and C as the major fatty acids. The genomic DNA G+C content was 65.6 mol%. Sequence data showed that strain CH15-11 was most closely related to Dae 20 (97.6 %), Gsoil 634 (97.2 %) and TDMA-16 (97.0 %). However, the DNA–DNA relatedness values between strain CH15-11 and the most closely related type strains were within a range of 35–59 %. Thus, based on the phylogenetic, phenotypic and genetic data, strain CH15-11 was classified as a member of the genus as a representative of a novel species, for which the name sp. nov. is proposed. The type strain is CH15-11 ( = KCTC 23718 = JCM 17887).

  • Published Online:
Funding
This study was supported by the:
  • Advanced Biomass Research Center, Global Frontier Program
  • Korean Ministry of Education, Science & Technology
  • Center for Aquatic Ecosystem Restoration (CAER)
  • Korean Ministry of Environment
© 2014 IUMS
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2014-04-01
2024-04-16
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Sphingomonas daechungensis sp. nov., isolated from sediment of a eutrophic reservoir
Int J Syst Evol Microbiol 64, 1412 (2014); https://doi.org/10.1099/ijs.0.054510-0
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