1887

Abstract

Three unknown Gram-stain-positive, catalase-negative, facultatively anaerobic and coccus-shaped strains of bacteria were isolated from the digestive tracts of wasps (). Analysis of 16S rRNA gene sequences revealed that these strains had identical sequences and showed that , with 96.2 % sequence similarity, was the closest phylogenetic neighbour. Further analyses based on and gene sequences of representatives of the family and genotypic and phenotypic characterization using (GTG)-PCR fingerprintings, RI ribotyping, DNA G+C content, whole-cell protein profiling, cellular fatty acid profiles analysis and extensive biotyping confirmed that the investigated strains were representatives of a novel bacterial species within the genus for which the name sp. nov. is proposed. The type strain is VOSTP2 ( = DSM 24756 = CCM 7946).

Funding
This study was supported by the:
  • Czech National Agency for Agricultural Research (Award NAZV QJ 1210047)
  • Institutional Research Concept (Award RVO 61388971)
  • Institutional Research Project of the Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic (Award RVO: 67985904)
  • Ministry of Education, Youth and Sports of the Czech Republic (Award CEB: CZ.1.07/2.3.00/20.0183)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.054940-0
2014-03-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/3/731.html?itemId=/content/journal/ijsem/10.1099/ijs.0.054940-0&mimeType=html&fmt=ahah

References

  1. Collins M. D. ( 2009 ). Genus Vagococcus . . In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 616618. Edited by Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  2. Collins M. D., Ash C., Farrow J. A., Wallbanks S., Williams A. M. ( 1989 ). 16S ribosomal ribonucleic acid sequence analyses of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov.. J Appl Bacteriol 67, 453460. [View Article] [PubMed]
    [Google Scholar]
  3. Dobson C. M., Deneer H., Lee S., Hemmingsen S., Glaze S., Ziola B. ( 2002 ). Phylogenetic analysis of the genus Pediococcus, including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer. . Int J Syst Evol Microbiol 52, 20032010. [View Article] [PubMed]
    [Google Scholar]
  4. Fischer W., Arneth-Seifert D. ( 1998 ). d-Alanylcardiolipin, a major component of the unique lipid pattern of Vagococcus fluvialis . . J Bacteriol 180, 29502957. [PubMed]
    [Google Scholar]
  5. Goh S. H., Facklam R. R., Chang M., Hill J. E., Tyrrell G. J., Burns E. C., Chan D., He C., Rahim T. & other authors ( 2000 ). Identification of Enterococcus species and phenotypically similar Lactococcus and Vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences. . J Clin Microbiol 38, 39533959. [PubMed]
    [Google Scholar]
  6. Hoyles L., Lawson P. A., Foster G., Falsen E., Ohlén M., Grainger J. M., Collins M. D. ( 2000 ). Vagococcus fessus sp. nov., isolated from a seal and a harbour porpoise. . Int J Syst Evol Microbiol 50, 11511154. [View Article] [PubMed]
    [Google Scholar]
  7. Jaffrès E., Prévost H., Rossero A., Joffraud J. J., Dousset X. ( 2010 ). Vagococcus penaei sp. nov., isolated from spoilage microbiota of cooked shrimp (Penaeus vannamei). . Int J Syst Evol Microbiol 60, 21592164. [View Article] [PubMed]
    [Google Scholar]
  8. Jeon Y. S., Chung H., Park S., Hur I., Lee J. H., Chun J. ( 2005 ). jphydit: a java-based integrated environment for molecular phylogeny of ribosomal RNA sequences. . Bioinformatics 21, 31713173. [View Article] [PubMed]
    [Google Scholar]
  9. Killer J., Kopecný J., Mrázek J., Rada V., Benada O., Koppová I., Havlík J., Straka J. ( 2009 ). Bifidobacterium bombi sp. nov., from the bumblebee digestive tract. . Int J Syst Evol Microbiol 59, 20202024. [View Article] [PubMed]
    [Google Scholar]
  10. Killer J., Kopečný J., Mrázek J., Havlík J., Koppová I., Benada O., Rada V., Kofroňová O. ( 2010 ). Bombiscardovia coagulans gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of bumblebees. . Syst Appl Microbiol 33, 359366. [View Article] [PubMed]
    [Google Scholar]
  11. Killer J., Kopečný J., Mrázek J., Koppová I., Havlík J., Benada O., Kott T. ( 2011 ). Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract. . Int J Syst Evol Microbiol 61, 13151321. [View Article] [PubMed]
    [Google Scholar]
  12. Killer J., Mrázek J., Bunešová V., Havlík J., Koppová I., Benada O., Rada V., Kopečný J., Vlková E. ( 2013 ). Pseudoscardovia suis gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of wild pigs (Sus scrofa). . Syst Appl Microbiol 36, 1116. [View Article] [PubMed]
    [Google Scholar]
  13. Lawson P. A., Foster G., Falsen E., Ohlén M., Collins M. D. ( 1999 ). Vagococcus lutrae sp. nov., isolated from the common otter (Lutra lutra). . Int J Syst Bacteriol 49, 12511254. [View Article] [PubMed]
    [Google Scholar]
  14. Lawson P. A., Falsen E., Cotta M. A., Whitehead T. R. ( 2007 ). Vagococcus elongatus sp. nov., isolated from a swine-manure storage pit. . Int J Syst Evol Microbiol 57, 751754. [View Article] [PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  16. Naser S., Thompson F. L., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Thompson C. C., Vancanneyt M., Swings J. ( 2005a ). Phylogeny and identification of Enterococci by atpA gene sequence analysis. . J Clin Microbiol 43, 22242230. [View Article] [PubMed]
    [Google Scholar]
  17. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. ( 2005b ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151, 21412150. [View Article] [PubMed]
    [Google Scholar]
  18. Pot B., Vandamme P., Kersters K. ( 1994 ). Analysis of electrophoretic whole-organism protein fingerprints. . In Modern Microbiological Methods: Chemical Methods in Prokaryotic Systematics, pp. 493521. Edited by Goodfellow M., O'Donnell A. G. . Chichester, UK:: John Wiley & Sons;.
    [Google Scholar]
  19. Rada V., Petr J. ( 2000 ). A new selective medium for the isolation of glucose non-fermenting bifidobacteria from hen caeca. . J Microbiol Methods 43, 127132. [View Article] [PubMed]
    [Google Scholar]
  20. Román L., Real F., Sorroza L., Padilla D., Acosta B., Grasso V., Bravo J., Acosta F. ( 2012 ). The in vitro effect of probiotic Vagococcus fluvialis on the innate immune parameters of Sparus aurata and Dicentrarchus labrax . . Fish Shellfish Immunol 33, 10711075. [View Article] [PubMed]
    [Google Scholar]
  21. Ronquist F., Teslenko M., van der Mark P., Ayres D. L., Darling A., Höhna S., Larget B., Liu L., Suchard M. A., Huelsenbeck J. P. ( 2012 ). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. . Syst Biol 61, 539542. [View Article] [PubMed]
    [Google Scholar]
  22. Ruiz-Zarzuela I., de Bias I., Gironés O., Ghittino C., Múazquiz J. L. ( 2005 ). Isolation of Vagococcus salmoninarum in rainbow trout, Oncorhynchus mykiss (Walbaum), broodstocks: characterization of the pathogen. . Vet Res Commun 29, 553562. [View Article] [PubMed]
    [Google Scholar]
  23. Scardovi V. ( 1986 ). Genus Bifidobacterium . . In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 14181434. Edited by Sneath P. H. A., Mair N. S., Sharp M. E., Holt J. G. . Baltimore:: Williams and Wilkins;.
    [Google Scholar]
  24. Shewmaker P. L., Steigerwalt A. G., Morey R. E., Carvalho M. G., Elliott J. A., Joyce K., Barrett T. J., Teixeira L. M., Facklam R. R. ( 2004 ). Vagococcus carniphilus sp. nov., isolated from ground beef. . Int J Syst Evol Microbiol 54, 15051510. [View Article] [PubMed]
    [Google Scholar]
  25. Smith H. L. Jr, Goodner K. ( 1958 ). Detection of bacterial gelatinases by gelatin-agar plate methods. . J Bacteriol 76, 662665. [PubMed]
    [Google Scholar]
  26. Sorroza L., Padilla D., Acosta F., Román L., Grasso V., Vega J., Real F. ( 2012 ). Characterization of the probiotic strain Vagococcus fluvialis in the protection of European sea bass (Dicentrarchus labrax) against vibriosis by Vibrio anguillarum . . Vet Microbiol 155, 369373. [View Article] [PubMed]
    [Google Scholar]
  27. Švec P., Nováková D., Zácková L., Kukletová M., Sedlácek I. ( 2008 ). Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans . . Antonie van Leeuwenhoek 94, 573579. [View Article] [PubMed]
    [Google Scholar]
  28. Švec P., Vandamme P., Bryndová H., Holochová P., Kosina M., Maslanová I., Sedlácek I. ( 2012 ). Enterococcus plantarum sp. nov., isolated from plants. . Int J Syst Evol Microbiol 62, 14991505. [View Article] [PubMed]
    [Google Scholar]
  29. Teixeira L. M., Carvalho M. G., Merquior V. L., Steigerwalt A. G., Brenner D. J., Facklam R. R. ( 1997 ). Phenotypic and genotypic characterization of Vagococcus fluvialis, including strains isolated from human sources. . J Clin Microbiol 35, 27782781. [PubMed]
    [Google Scholar]
  30. Ventura M., Canchaya C., Del Casale A., Dellaglio F., Neviani E., Fitzgerald G. F., van Sinderen D. ( 2006 ). Analysis of bifidobacterial evolution using a multilocus approach. . Int J Syst Evol Microbiol 56, 27832792. [View Article] [PubMed]
    [Google Scholar]
  31. Vlková E., Rada V., Popelářová P., Trojanová I., Killer J. ( 2006 ). Antimicrobial susceptibility of bifidobacteria isolated from gastrointestinal tract of calves. . Livest Sci 105, 253259. [View Article]
    [Google Scholar]
  32. Wallbanks S., Martinez-Murcia A. J., Fryer J. L., Phillips B. A., Collins M. D. ( 1990 ). 16S rRNA sequence determination for members of the genus Carnobacterium and related lactic acid bacteria and description of Vagococcus salmoninarum sp. nov.. Int J Syst Bacteriol 40, 224230. [View Article] [PubMed]
    [Google Scholar]
  33. Wang L., Cui Y. S., Kwon C. S., Lee S. T., Lee J. S., Im W. T. ( 2011 ). Vagococcus acidifermentans sp. nov., isolated from an acidogenic fermentation bioreactor. . Int J Syst Evol Microbiol 61, 11231126. [View Article] [PubMed]
    [Google Scholar]
  34. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703. [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.054940-0
Loading
/content/journal/ijsem/10.1099/ijs.0.054940-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error