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Volume 64, Issue Pt_1

gen. nov., sp. nov., a novel group 4 thermophilic member of the phylum from geothermal soils Free

Abstract

An aerobic, thermophilic, moderately acidophilic non-spore-forming bacterium, strain K22, was isolated from geothermally heated soil at Mount Ngauruhoe, New Zealand. On the basis of 16S rRNA gene sequence similarity, K22 was shown to belong to subdivision 4 of the phylum and to be most closely related to ‘ Chloracidobacterium thermophilum’ (86 %) and (86 %). Cells stained Gram-negative and were catalase and oxidase-positive. The major fatty acids detected were iso-C, iso-C, iso-C and iso-C when standard lipid extraction protocols were employed. Analysis of the total cell lipid acid hydrolysate also detected membrane-spanning and ether lipids, which made up approximately 40 % of the total membrane composition. These lipids included dicarboxylic (iso-diabolic) acid and the glyceryl ether of alkyl analogues of iso-C and iso-diabolic acid. The G+C content of the genomic DNA was 59.6 mol% and the primary respiratory quinone was MK-8. Strain K22 grew at 50–69 °C with an optimum temperature of 65 °C and at pH 4.1–7.8 with an optimum growth pH of 6.5. NaCl tolerance was up to 1 % (w/v). Cells displayed a chemoheterotrophic and obligately aerobic metabolism. Cells grew on nutrient broth, alginate, arabinose, Casamino acids, glucose, lactate, formate, mannose, sodium alginate, peptone, sucrose, tryptone, xanthan, xylan, xylose and yeast extract. Nitrogen sources included nitrate, ammonium, urea, yeast extract and Casamino acids, but not dinitrogen gas. The distinct phylogenetic position and the phenotypic characteristics separate strain K22 from all other members of the class and indicate that it represents a novel species and genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is K22 ( = DSM 25857 = ICMP 18710).

  • Published Online:
Funding
This study was supported by the:
  • Geothermal Resources of New Zealand (GRN) Programme
  • GNS Science
  • Wairakei Environmental Mitigation Charitable Trust (WEMCT)
© 2014 GNS Science

Erratum

An erratum has been published for this content:
gen. nov., sp. nov., a novel group 4 thermophilic member of the phylum from geothermal soils
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2014-01-01
2024-04-18
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Pyrinomonas methylaliphatogenes gen. nov., sp. nov., a novel group 4 thermophilic member of the phylum Acidobacteria from geothermal soils
Int J Syst Evol Microbiol 64, 220 (2014); https://doi.org/10.1099/ijs.0.055079-0
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