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Volume 63, Issue Pt_12

sp. nov., a marine bacterium isolated from a tidal flat Free

Abstract

A Gram-stain-negative, non-spore-forming, facultatively anaerobic bacterium, designated strain SC18, was isolated from a tidal flat of Suncheon bay in South Korea. Cells were rod-shaped and motile by means of a polar flagellum. Cells were catalase-, oxidase- and β-haemolysis-positive. Growth was observed at 4–37 °C (optimum, 25–30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and in the presence of 0–5.0 % (w/v) NaCl (optimum, 0–2 %). The major cellular fatty acids were summed feature 3 (comprising Cω7 and/or Cω6), iso-C and C. The polar lipid pattern indicated the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminolipid and three unidentified lipids. Strain SC18 contained Q-7, Q-8, MK-7 and MMK-7 as the dominant respiratory quinones and the G+C content of the genomic DNA was 41.3 mol%. Phylogenetic analysis based on 16S rRNA and gyrase B () gene sequences showed that strain SC18 formed a tight phyletic lineage with members of the genus . Strain SC18 was related most closely to OS217 (97.3 % 16S rRNA gene sequence similarity) and TF-27 (97.1 %), but the DNA–DNA relatedness levels between strain SC18 and the type strains of and were 18.3±2.8 and 22.5±1.6 % (mean±), respectively. On the basis of phenotypic, chemotaxonomic and molecular features, strain SC18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SC18 ( = KACC 16187 = JCM 17801).

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Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2012R1A1B)
  • National Institute of Biological Resources (Award 2013-02-001)
© 2013 IUMS
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2013-12-01
2024-04-18
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Shewanellaaestuarii sp. nov., a marine bacterium isolated from a tidal flat
Int J Syst Evol Microbiol 63, 4683 (2013); https://doi.org/10.1099/ijs.0.055178-0
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