1887

Abstract

Two thermophilic bacterial strains, designated YIM 77925 and YIM 77777, were isolated from two hot springs, one in the Hydrothermal Explosion (Shuirebaozhaqu) area and Frog Mouth Spring in Tengchong county, Yunnan province, south-western China. The taxonomic positions of the two isolates were investigated by a polyphasic approach. Cells of the two strains were Gram-stain-negative, aerobic and rod-shaped. They were able to grow at 50–70 °C, pH 6.0–8.0 and with a NaCl tolerance up to 0.5 % (w/v). Colonies are circular, convex, non-transparent and produce yellow pigment. Phylogenetic analyses based on 16S rRNA gene sequences comparison clearly demonstrated that strains YIM 77925 and YIM 77777 represent members of the genus , and they also detected low-level similarities of 16S rRNA gene sequences (below 97 %) compared with all other species in this genus. Their predominant menaquinone was MK-8. The genomic DNA G+C contents of strains YIM 77925 and YIM 77777 were 65.6 mol% and 67.2 mol%, respectively. Based on the results of physiological and biochemical tests and phylogenetic analyses, strains YIM 77925 and YIM 77777 could not be classified as representing any species of the genus with a validly published name. Thus the two strains are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 77925 ( = DSM 25901 = CCTCC 2012061).

Funding
This study was supported by the:
  • Key Project of International Cooperation of China Ministry of Science & Technology (MOST) (Award 2013DFA31980)
  • Natural Science Foundation of China (Award 31070007)
  • Key Project of Yunnan Provincial Natural Science Foundation (Award 2013FA004)
  • Research Project of Education Department of Henan Province of China (Award 2011A180025)
  • Henan Provincial Undergraduate Training Programs for Innovation and Entrepreneurship (Award 2011060)
  • National Science Foundation (Award OISE-0968421)
  • Chinese Academy of Sciences
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2014-02-01
2024-03-28
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