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Volume 64, Issue Pt_3

sp. nov., isolated from deep freshwater sediment Free

Abstract

The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or non-motile (stalked) and rod-shaped bacteria, DS48-5-2 and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the two isolates had clear affiliation with and the closest relatedness to FWC 38, ATCC 15257 and H-E3-2 showing 98.5 %, 97.3 % and 97.3 % 16S rRNA gene sequence similarity, respectively, and 96.1–96.7 % similarity to all other species of the genus . The two isolates shared 100 % 16S rRNA gene sequence similarity. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (Cω6 and/or Cω7), C, Cω7 11-methyl and summed feature 3 (Cω6 and/or Cω7). The G+C contents of the genomic DNA of strains DS48-5-2 and DS48-6-3 were 66.7 mol% and 66.2 mol%, respectively. DNA–DNA hybridization values of strains DS48-5-2 and DS48-6-3 with FWC 38, ATCC 15257 and H-E3-2 were 19.3 %–24.4 %. Thus, based on the evidence from polyphasic studies, it is proposed that strains DS48-5-2 and DS48-6-3 are representatives of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is DS48-5-2 ( = KCTC 32480 = JCM 19440).

  • Published Online:
Funding
This study was supported by the:
  • Advanced Biomass R&D Centre
  • Global Frontier Program funded by the Korean Ministry of Education, Science & Technology
  • CAER (Centre for Aquatic Ecosystem Restoration)
  • Eco-STAR project from the Korean Ministry of Environment
  • KIST Institutional Program (Award  2E24280)
© 2014 IUMS
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2014-03-01
2024-04-19
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Caulobacterprofunda sp. nov., isolated from deep freshwater sediment
Int J Syst Evol Microbiol 64, 762 (2014); https://doi.org/10.1099/ijs.0.057240-0
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