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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_3

sp. nov., an endophytic bacterium isolated from roots of Roxb. Free

Abstract

A Gram-staining-positive, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated GTGR-8, which formed white colonies, was isolated from roots of Roxb. collected from Taibai Mountain in Shaanxi Province, north-west China. Strain GTGR-8 grew optimally at 28–30 °C, at pH 7.0–8.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTGR-8 was a member of the genus and was closely related to B33D1 (98.9 % similarity), BXN5-15 (97.0 %) and Gsoil 355 (96.9 %). No other recognized bacterial species showed more than 94.2 % 16S rRNA gene sequence similarity to the novel isolate. The only respiratory quinone of strain GTGR-8 was MK-7(H) and the major fatty acids (>5 %) were iso-C, Cω9, Cω8, Cω6 (6,9,12) and Cω6. The DNA GC content was 71.0 mol%. DNA–DNA relatedness for strain GTGR-8 with respect to its closest relatives, KCTC 9974 and KCTC 19420, was 52.5 and 24.5 %, respectively. Based on phenotypic, phylogenetic and genotypic data, strain GTGR-8 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is GTGR-8 ( = CCTCC AB 2013011 = KCTC 29190).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31100001)
  • Natural Science Foundation of Shaanxi Province, China (Award 2012JQ3006)
© 2014 IUMS
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2014-03-01
2024-04-19
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Solirubrobacter phytolaccae sp. nov., an endophytic bacterium isolated from roots of Phytolacca acinosa Roxb.
Int J Syst Evol Microbiol 64, 858 (2014); https://doi.org/10.1099/ijs.0.057554-0
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