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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_6

Molecular signatures for members of the genus and the class Free

Abstract

The bacteria belonging to the class , due to their ability to dehalogenate chlorinated compounds, are of much interest for bioremediation of contaminated sites. We report here comparative analyses on different genes/proteins from the genomes of members of the class These studies have identified numerous novel molecular markers in the forms of conserved signature indels (CSIs) in broadly distributed proteins and conserved signature genes/proteins (CSPs), which are uniquely found in members of the class , but except for an isolated exception, they are not found in other sequenced bacterial genomes. Of these molecular markers, nine CSIs in divergent proteins and 19 CSPs are specific for members of the genera and , providing potential molecular markers for the bacterial class . Additionally, four CSIs in divergent proteins and 28 CSPs are only found in all members of the genus for which genome sequences are available, but they are absent in and in other bacteria. The gene sequences of several of these CSPs exhibiting specificity for the genus or the class are highly conserved and PCR primers based upon them provide a novel means for identification of other related bacteria. Two other CSIs identified in this study in the SecD and aspartate carbomyltransferase proteins weakly support an affiliation of the class with the other members of the phylum

  • Published Online:
Funding
This study was supported by the:
  • MRI-ORF
  • Water Round
© 2014 IUMS
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2014-06-01
2024-04-26
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Molecular signatures for members of the genus Dehalococcoides and the class Dehalococcoidia
Int J Syst Evol Microbiol 64, 2176 (2014); https://doi.org/10.1099/ijs.0.057919-0
/content/journal/ijsem/10.1099/ijs.0.057919-0
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