1887

Abstract

Strain JC207 was isolated from a deep (265 m) sea sediment, and appeared as dark yellow colonies on agar plates with cells staining Gram-negative. Catalase, oxidase and caseinase were positive, while chitinase, gelatinase and amylase were negative. Major (>5 %) fatty acids were iso-C, anteiso-C, iso-Cω9, iso-C, iso-C 3-OH, iso-C 3-OH, iso-C and iso-CG. Strain JC207 contained phosphatidylethanolamine as the major polar lipid, with minor amounts of five unidentified lipids. A bacterial hopane derivative, diplopterol and adenosylhopane were the major hopanoids. Genomic DNA G+C content was 47.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC207 represented a member of the genus within the family of the phylum . Strain JC207 had sequence similarity with YIM-C338 (98 %), BH206 (97.6 %) and other members of the genus (<96.8 %). However, strain JC207 showed an average of 23.6±4 and 37±4 relatedness (based on DNA–DNA hybridization) with CGMCC 1.6308 ( = YIM-C338) and KCTC 12883 ( = BH206), respectively. Morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC207 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JC207 ( = KCTC 32444 = CGMCC 1.12641).

Funding
This study was supported by the:
  • Council of Scientific and Industrial Research (CSIR)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058149-0
2014-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/3/984.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058149-0&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  2. Chakravarthy S. K., Ramaprasad E. V. V., Shobha E., Sasikala Ch., Ramana Ch. V. ( 2012 ). Rhodoplanes piscinae sp. nov. isolated from pond water. . Int J Syst Evol Microbiol 62, 28282834. [View Article] [PubMed]
    [Google Scholar]
  3. Chen Y. G., Cui X. L., Zhang Y.-Q., Li W. J., Wang Y. X., Kim C. J., Lim J.-M., Xu L.-H., Jiang C.-L. ( 2008 ). Salinimicrobium terrae sp. nov., isolated from saline soil, and emended description of the genus Salinimicrobium . . Int J Syst Evol Microbiol 58, 25012504. [View Article] [PubMed]
    [Google Scholar]
  4. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  5. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  6. Lee S. Y., Park S., Oh T. K., Yoon J. H. ( 2012 ). Salinimicrobium gaetbulicola sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 62, 10271031. [View Article] [PubMed]
    [Google Scholar]
  7. Lim J.-M., Jeon C. O., Lee S. S., Park D.-J., Xu L.-H., Jiang C.-L., Kim C.-J. ( 2008 ). Reclassification of Salegentibacter catena Ying et al. 2007 as Salinimicrobium catena gen. nov., comb. nov. and description of Salinimicrobium xinjiangense sp. nov., a halophilic bacterium isolated from Xinjiang province in China. . Int J Syst Evol Microbiol 58, 438442. [View Article] [PubMed]
    [Google Scholar]
  8. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  9. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H. P. ( 2013 ). When should a DDH experiment be mandatory in microbial taxonomy. ? Arch Microbiol 195, 413418. [View Article] [PubMed]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  11. Nedashkovskaya O. I., Vancanneyt M., Kim S. B., Han J., Zhukova N. V., Shevchenko L. S. ( 2010 ). Salinimicrobium marinum sp. nov., a halophilic bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Salinimicrobium and Salinimicrobium catena . . Int J Syst Evol Microbiol 60, 23032306. [View Article] [PubMed]
    [Google Scholar]
  12. Ramana V. V., Sasikala C., Ramaprasad E. V. V., Ramana C. V. ( 2010 ). Description of Ectothiorhodospira salini sp. nov.. J Gen Appl Microbiol 56, 313319. [View Article] [PubMed]
    [Google Scholar]
  13. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Evol Microbiol 44, 846849. [View Article]
    [Google Scholar]
  14. Subhash Y., Sasikala Ch., Ramana Ch. V. ( 2013a ). Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. . Int J Syst Evol Microbiol 63, 34633469. [View Article] [PubMed]
    [Google Scholar]
  15. Subhash Y., Tushar L., Sasikala Ch., Ramana ChV. ( 2013b ). Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. . Int J Syst Evol Microbiol 63, 45244532. [View Article] [PubMed]
    [Google Scholar]
  16. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V. ( 2013c ). Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. . Int J Syst Evol Microbiol 63, 21322137. [View Article] [PubMed]
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  18. Trüper H. G., Pfennig N. ( 1981 ). Isolation of members of the families Chromatiaceae and Chlorobiaceae . . In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 279289. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  19. Ying J.-Y., Liu Z.-P., Wang B.-J., Dai X., Yang S.-S., Liu S.-J. ( 2007 ). Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter . . Int J Syst Evol Microbiol 57, 219222. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.058149-0
Loading
/content/journal/ijsem/10.1099/ijs.0.058149-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error