@article{mbs:/content/journal/ijsem/10.1099/ijs.0.058487-0, author = "Kato, Shingo and Krepski, Sean and Chan, Clara and Itoh, Takashi and Ohkuma, Moriya", title = "Ferriphaselus amnicola gen. nov., sp. nov., a neutrophilic, stalk-forming, iron-oxidizing bacterium isolated from an iron-rich groundwater seep", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_3", pages = "921-925", doi = "https://doi.org/10.1099/ijs.0.058487-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.058487-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A neutrophilic, stalk-forming, iron-oxidizing bacterium, strain OYT1T, which was isolated from a groundwater seep in Ohyato Park, Tokyo, Japan, was subjected to taxonomic analysis. OYT1T was a motile, bean-shaped, Gram-negative bacterium that was able to grow at 8–30 °C (optimally at 25–30 °C) and at pH 5.6–7.3 (optimally at pH 6.1–6.5). The strain grew microaerobically and autotrophically. Major cellular fatty acids detected were C16 : 1ω7c/C16 : 1ω6c and C16 : 0. The total DNA G+C content was 57.6 mol%. 16S rRNA gene sequence analysis revealed that strain OYT1T was affiliated with the class Betaproteobacteria and clustered with iron-oxidizing bacteria isolated from groundwater seeps and wetlands and with uncultured clones detected in freshwater iron-rich environments. Based on the phenotypic and phylogenetic characteristics of strain OYT1T, we propose that the strain represents a novel species in a new genus, for which the name Ferriphaselus amnicola gen. nov., sp. nov. is proposed; the type strain of Ferriphaselus amnicola is OYT1T ( = JCM 18545T = DSM 26810T).", }