Pedobacter nutrimenti sp. nov., isolated from chilled food
Derichs, Julia and Kämpfer, Peter and Lipski, André,, 64, 1310-1316 (2014),
doi = https://doi.org/10.1099/ijs.0.058677-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-stain-negative, aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated J22T, was isolated from fresh pasta filled with pork (tortellini) that had been stored at 6 °C. Based on 16S rRNA gene sequence analysis, strain J22T falls within the radiation of species of the genus Pedobacter . On the basis of 16S rRNA gene sequence similarities, the closest species phylogenetically are Pedobacter panaciterrae LMG 23400T (98.7 %), Pedobacter africanus DSM 12126T (98.5 %) and Pedobacter heparinus DSM 2366T (98.3 %). The only isoprenoid quinone of strain J22T was menaquinone 7 (MK-7). The major fatty acids were iso-C15 : 0 (34.8 %), C16 : 1 cis9 (24.9 %), C16 : 0 (7.3 %), C14 : 0 (4.8 %) and iso-C17 : 0 3-OH (4.4 %). These chemotaxonomic characteristics supported the assignment of the isolate to the genus Pedobacter . DNA–DNA hybridization experiments showed that the DNA–DNA relatedness between strain J22T and its closest phylogenetic neighbour, Pedobacter panaciterrae LMG 23400T, was less than 36 %. This indicated that the isolate was distinct from this species. Biochemical tests allowed strain J22T to be phenotypically differentiated from closely related species of the genus Pedobacter . As a consequence, strain J22T should be classified as a representative of a novel species of the genus Pedobacter for which the name Pedobacter nutrimenti sp. nov. is proposed. The type strain is J22T ( = DSM 27372T = CCUG 64422T).,
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