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Volume 64, Issue Pt_3

sp. nov., a marine bacterium isolated from a coastal-marine wetland Free

Abstract

A novel Gram-stain-negative, slightly halophilic bacterium, designated strain GASx41, was isolated from soil of the coastal–marine wetland Gomishan in Iran. Cells of strain GASx41 were curved, ring-like or horseshoe-shaped rods and non-motile. Strain GASx41 was strictly aerobic, and catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 1–10 % (w/v), with optimum growth occurring at 2.5–3 % (w/v) NaCl. The optimum temperature and pH for growth were 25–30 °C and pH 7.5–8.0. On the basis of 16S rRNA gene sequence analysis, strain GASx41 was shown to belong to the genus within the phylum and showed closest phylogenetic similarity to ‘’ HMD3055 (98.0 %). The DNA G+C content of strain GASx41 was 48.1 mol%. The major cellular fatty acids of strain GASx41 were iso-C, summed feature 4 (iso-C 2-OH and/or Cω7), anteiso-C 2-OH, anteiso-C and iso-C 3-OH, and its polar lipid pattern consisted of phosphatidylethanolamine, phosphatidylcholine and 12 unknown lipids. The only quinone present was menaquinone 7 (MK-7). All these features confirmed the placement of isolate GASx41 within the genus . On the basis of evidence from this study, a novel species of the genus sp. nov., is proposed, with strain GASx41 ( = IBRC-M 10761 = CECT 8341) as the type strain.

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Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1391-15)
  • Spanish Ministerio de Economía y Competitividad (Award CGL2010-19303)
  • FEDER
  • Junta de Andalucía (Award P10-CVI-6226)
© 2014 IUMS
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2014-03-01
2024-04-16
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Cyclobacterium halophilum sp. nov., a marine bacterium isolated from a coastal-marine wetland
Int J Syst Evol Microbiol 64, 1000 (2014); https://doi.org/10.1099/ijs.0.058743-0
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