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Volume 64, Issue Pt_4

sp. nov. isolated from mangrove sediment Free

Abstract

A novel bacterium, strain MUSC 273, was isolated from mangrove sediments of the Tanjung Lumpur river in the state of Pahang in peninsular Malaysia. The bacterium was yellow-pigmented, Gram-negative, rod-shaped and non-spore-forming. The taxonomy of strain MUSC 273 was studied by a polyphasic approach and the organism showed a range of phenotypic and chemotaxonomic properties consistent with those of the genus . The 16S rRNA gene sequence of strain MUSC 273 showed the highest sequence similarity to those of H25 (96.8 %), TUT562 (96.4 %) and CC-TPE-1 (95.9 %) and lower sequence similarity to members of all other species of the genus . Furthermore, in phylogenetic analyses based on the 16S rRNA gene sequence, strain MUSC 273 formed a distinct cluster with members of the genus . DNA–DNA relatedness of strain MUSC 273 to the type strains of the most closely related species, MCCC 1A01080 and DSM 18518, was 29.2 % (reciprocal 31.0 %) and 17 % (reciprocal 18 %), respectively. The major respiratory quinone was ubiquinone Q-10, the major polyamine was spermidine and the DNA G+C content was 63.3±0.1 mol%. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine and sphingoglycolipid. The major fatty acids were Cω7, Cω6, C, C 2-OH and Cω7. Comparison of BOX-PCR fingerprints indicated that strain MUSC 273 represented a unique DNA profile. The combined genotypic and phenotypic data showed that strain MUSC 273 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MUSC 273 ( = DSM 27798 = MCCC 1A00645 = NBRC 109947).

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Funding
This study was supported by the:
  • University of Malaya High Impact Research Grant
  • UM-MOHE HIR Nature Microbiome grant (Award H-50001-A000027)
  • External Industry Grant (Award GBA-808138)
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2014-04-01
2024-04-18
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Novosphingobium malaysiense sp. nov. isolated from mangrove sediment
Int J Syst Evol Microbiol 64, 1194 (2014); https://doi.org/10.1099/ijs.0.059014-0
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