1887

Abstract

A Gram-stain-positive, short rod-shaped and motile, mildly halotolerant, endospore-forming bacterium, FJAT-13985, was isolated from the internal tissues of root. Strain FJAT-13985 grew at 20–45 °C (optimum 30 °C) and pH 5.7–9.0 (optimum pH 7.0) and in the presence of 0–2 % (w/v) NaCl [optimum 1 % (w/v)]. The strain was catalase-positive and oxidase-negative. The cell wall of strain FJAT-13985 contained -diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (97.4 %). The major fatty acids of the strain were anteiso-C (23.3 %) and iso-C (40.8 %). The DNA G+C content was 41.64 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FJAT-13985 is a member of the genus and is most closely related to DSM 15600 (98.4 %), DSM 15602 (98.2 %) and DSM 15603 (98.3 %). DNA–DNA hybridization indicated that relatedness between strain FJAT-13985 and its closest relative, DSM 15600, was 36.63 %. The phenotypic, chemotaxonomic and genotypic properties clearly indicate that strain FJAT-13985 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJAT-13985 ( = DSM 25968 = CGMCC1.12238).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (NSFC) (Award 31370059)
  • Chinese Ministry of Agriculture (Award 948 project (2011-G25))
  • Project of Agriculture Science and Technology achievement transformation (Award 2010GB2C400220)
  • Chinese Ministry of Science and Technology (Award 2012DFA31120)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.059485-0
2014-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/10/3346.html?itemId=/content/journal/ijsem/10.1099/ijs.0.059485-0&mimeType=html&fmt=ahah

References

  1. Albuquerque L., Tiago I., Taborda M., Nobre M. F., Veríssimo A., da Costa M. S. ( 2008 ). Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond. . Int J Syst Evol Microbiol 58, 226230. [View Article] [PubMed]
    [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. ( 1991 ). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. . Lett Appl Microbiol 13, 202206. [View Article]
    [Google Scholar]
  3. Atlas R. M. ( 1993 ). Handbook of Microbiological Media. Edited by Parks L. C. . Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  4. Bibi F., Chung E. J., Jeon C. O., Chung Y. R. ( 2011 ). Bacillus graminis sp. nov., an endophyte isolated from a coastal dune plant. . Int J Syst Evol Microbiol 61, 15671571. [View Article] [PubMed]
    [Google Scholar]
  5. Chen Y. G., Cui X. L., Pukall R., Li H. M., Yang Y. L., Xu L. H., Wen M. L., Peng Q., Jiang C. L. ( 2007 ). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. . Int J Syst Evol Microbiol 57, 23272332. [View Article] [PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  7. Dong X., Cai M. ( 2001 ). Manual of systematic and determinative bacteriology. . In: Science Press, Beijing. (In Chinese). [View Article] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  9. Goodfellow M., Stainsby F. M., Davenport R., Chun J., Curtis T. ( 1998 ). Activated sludge foaming: the true extent of actinomycete diversity. . Water Sci Technol 37, 511519. [View Article]
    [Google Scholar]
  10. Gregersen T. ( 1978 ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5, 123127. [View Article]
    [Google Scholar]
  11. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996 ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46, 234239. [View Article] [PubMed]
    [Google Scholar]
  12. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29, 319322. [View Article]
    [Google Scholar]
  13. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodríguez-Díaz M., Felske A., De Vos P. ( 2004 ). Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. . Int J Syst Evol Microbiol 54, 4757. [View Article] [PubMed]
    [Google Scholar]
  14. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. (editors) ( 1985 ). Genetic Manipulation of Streptomyces. A Laboratory Manual. Norwich:: John Innes Foundation;.
    [Google Scholar]
  15. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  16. Jahnke K. D. ( 1992 ). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. . J Microbiol Methods 15, 6173. [View Article]
    [Google Scholar]
  17. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  18. Kämpfer P. ( 1994 ). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. . Syst Appl Microbiol 17, 8698. [View Article]
    [Google Scholar]
  19. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [View Article]
    [Google Scholar]
  20. Kanso S., Greene A. C., Patel B. K. ( 2002 ). Bacillus subterraneus sp. nov., an iron- and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer. . Int J Syst Evol Microbiol 52, 869874. [View Article] [PubMed]
    [Google Scholar]
  21. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  22. Ko K. S., Oh W. S., Lee M. Y., Lee J. H., Lee H., Peck K. R., Lee N. Y., Song J.-H. ( 2006 ). Bacillus infantis sp. nov. and Bacillus idriensis sp. nov., isolated from a patient with neonatal sepsis. . Int J Syst Evol Microbiol 56, 25412544. [View Article] [PubMed]
    [Google Scholar]
  23. Logan N. A., De Vos P. ( 2009 ). Genus I. Bacillus. Cohn 1872, 174AL . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 21128. Edited by De Vos P., Garrity G. M, Jones D., Krieg N. R., Ludwig W., Rainey F. A, Schleifer K.-H, Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  24. Logan N. A., Lebbe L., Hoste B., Goris J., Forsyth G., Heyndrickx M., Murray B. L., Syme N., Wynn-Williams D. D., De Vos P. ( 2000 ). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov.. Int J Syst Evol Microbiol 50, 17411753.[PubMed]
    [Google Scholar]
  25. Logan N. A., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodríguez-Díaz M., Heyndrickx M., De Vos P. ( 2004 ). Bacillus shackletonii sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. . Int J Syst Evol Microbiol 54, 373376. [View Article] [PubMed]
    [Google Scholar]
  26. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [View Article] [PubMed]
    [Google Scholar]
  27. Priest F. G., Goodfellow M., Todd C. ( 1988 ). A numerical classification of the genus Bacillus . . J Gen Microbiol 134, 18471882.[PubMed]
    [Google Scholar]
  28. Reva O. N., Smirnov V. V., Pettersson B., Priest F. G. ( 2002 ). Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.). . Int J Syst Evol Microbiol 52, 101107.[PubMed]
    [Google Scholar]
  29. Rössler D., Ludwig W., Schleifer K. H., Lin C., McGill T. J., Wisotzkey J. D., Jurtshuk P. Jr, Fox G. E. ( 1991 ). Phylogenetic diversity in the genus Bacillus as seen by 16S rRNA sequencing studies. . Syst Appl Microbiol 14, 266269. [View Article] [PubMed]
    [Google Scholar]
  30. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  31. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC News 20, 16.
    [Google Scholar]
  32. Seiler H., Schmidt V., Wenning M., Scherer S. ( 2012 ). Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site. . Int J Syst Evol Microbiol 62, 10921097. [View Article] [PubMed]
    [Google Scholar]
  33. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  34. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  35. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J. & other authors ( 2002 ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52, 10431047. [View Article] [PubMed]
    [Google Scholar]
  36. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  37. Ten L. N., Baek S. H., Im W. T., Larina L. L., Lee J. S., Oh H. M., Lee S. T. ( 2007 ). Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 57, 25322537. [View Article] [PubMed]
    [Google Scholar]
  38. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  39. Tiago I., Pires C., Mendes V., Morais P. V., da Costa M. S., Veríssimo A. ( 2006 ). Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater. . Int J Syst Evol Microbiol 56, 25712574. [View Article] [PubMed]
    [Google Scholar]
  40. Vaishampayan P., Probst A., Krishnamurthi S., Ghosh S., Osman S., McDowall A., Ruckmani A., Mayilraj S., Venkateswaran K. ( 2010 ). Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. . Int J Syst Evol Microbiol 60, 10311037. [View Article] [PubMed]
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  42. Xu D., Côté J. C. ( 2003 ). Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3′ end 16S rDNA and 5′ end 16S-23S ITS nucleotide sequences. . Int J Syst Evol Microbiol 53, 695704. [View Article] [PubMed]
    [Google Scholar]
  43. Yoon J. H., Kang S. S., Lee K. C., Kho Y. H., Choi S. H., Kang K. H., Park Y. H. ( 2001 ). Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood. . Int J Syst Evol Microbiol 51, 10871092. [View Article] [PubMed]
    [Google Scholar]
  44. Zhang J., Wang J. W., Fang C. Y., Song F., Xin Y. H., Qu L., Ding K. ( 2010 ). Bacillus oceanisediminis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 60, 29242929. [View Article] [PubMed]
    [Google Scholar]
  45. Zhang Y. Z., Chen W. F., Li M., Sui X. H., Liu H. C., Zhang X. X., Chen W. X. ( 2012 ). Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root. . Int J Syst Evol Microbiol 62, 359363. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.059485-0
Loading
/content/journal/ijsem/10.1099/ijs.0.059485-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error