1887

Abstract

A Gram-stain-positive, strictly aerobic, rod-shaped, non-motile bacterium, designated strain XH274, was isolated from a deep sub-seafloor sediment sample collected from the South Pacific Gyre (41° 58′ S 163° 11′ W) during the Integrated Ocean Drilling Program (IODP) Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH274 belonged to the genus and shared the highest 16S rRNA gene sequence similarity with KSL-2 (96.81 %), MDN22 (96.74 %) and RHLT2-1 (96.61 %). The DNA G+C content of strain XH274 was 74.6 mol%. The cell wall of strain XH274 contained -2,6-diaminopimelic acid as the diagnostic diamino acid, and ribose, glucose, galactose and mannose as the major whole-cell sugars. The major fatty acids were iso-C, Cω9 and Cω8. The major respiratory quinone was menaquinone-8(H). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids. On the basis of data from the polyphasic analysis, strain XH274 is considered to represent a novel species in the genus , for which the name Nocardioides sp. nov. is proposed. The type strain is XH274 ( = DSM 27278 = JCM 19260).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41276141)
  • National High Technology Research & Development Program of China (Award 2012AA091605)
  • China Ocean Mineral Resources Research & Development Association (Award DY125-15-R-03)
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2014-07-01
2024-03-29
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