Recommended minimal standards for description of new taxa of the genera Bifidobacterium, Lactobacillus and related genera
Mattarelli, Paola and Holzapfel, Wilhelm and Franz, Charles M. A. P. and Endo, Akihito and Felis, Giovanna E. and Hammes, Walter and Pot, Bruno and Dicks, Leon and Dellaglio, Franco,, 64, 1434-1451 (2014),
doi = https://doi.org/10.1099/ijs.0.060046-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Minimal standards for the description of new cultivable strains that represent novel genera and species belonging to the genera Bifidobacterium , Lactobacillus and related genera are proposed in accordance with Recommendation 30b of the Bacteriological Code (1990 Revision): the description of novel species should be based on phenotypic, genotypic and ecological characteristics to ensure a rich polyphasic characterization. Concerning genotypic characterization, in addition to DNA G+C content (mol%) data, the description should be based on DNA–DNA hybridization (DDH), 16S rRNA gene sequence similarities and at least two housekeeping gene (e.g. hsp60 and recA) sequence similarities. DDH might not be needed if the 16S rRNA gene sequence similarity to the closest known species is lower than 97 %. This proposal has been endorsed by members of the Subcommittee on the Taxonomy of Bifidobacterium , Lactobacillus and related organisms of the International Committee on the Systematics of Prokaryotes.,
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