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Volume 64, Issue Pt_9

sp. nov., an endophytic bacterium isolated from Free

Abstract

An endophytic bacterium, designated K-38, was isolated from the storage liquid in the stems of trees at the ancient Ugan River in Xinjiang, PR China. Strain K-38 was found to be rod-shaped, Gram-stain-negative, aerobic, non-motile and non-spore-forming. Strain K-38 grew at temperatures of 25–37 °C (optimum, 28 °C), at pH 6.0–9.0 (optimum, pH 7.5) and in the presence of 0–3 % (w/v) NaCl with 1 % as the optimum concentration for growth. According to phylogenetic analysis based on 16S rRNA gene sequences, strain K-38 was assigned to the genus with highest 16S rRNA gene sequence similarity of 97.2 % to W3, followed by 39/7 (96.5 %) and DN316 (96.2 %). Phylogenetic analysis of strain K-38 based on the protein coding genes , and confirmed (similarities were less than 90 %) it to be a representative of a distinctly delineated species of the genus . The DNA G+C content was determined to be 63.5 mol%. DNA–DNA relatedness between K-38 and W3 was 48.4 %, indicating genetic separation of strain K-38 from the latter strain. The major components of the cellular fatty acids in strain K-38 were revealed to be summed feature 8 (comprising Cω7 and/or Cω6; 57.2 %), C (13.6 %) and summed feature 2 (comprising C aldehyde, C 3-OH/iso-C I and/or unknown ECL 10.928; 11.0 %). Polar lipids of strain K-38 include phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipids and two unidentified phospholipids. Q-10 was the major quinone in strain K-38. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain K-38 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K-38 ( = CCTCC AB 2013068 = NRRL B-59990 = JCM 19159).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31060002 and U1203101)
  • KJZJ Foundation of Xinjiang Uyghur autonomous region (Award 201091236)
© 2014 IUMS
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2014-09-01
2024-04-19
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Rhizobiumpopuli sp. nov., an endophytic bacterium isolated from Populus euphratica
Int J Syst Evol Microbiol 64, 3215 (2014); https://doi.org/10.1099/ijs.0.061416-0
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