1887

Abstract

A novel orange-pigmented strain, designated R2-35, was isolated from a glacier till near Ny-Alesund, Svalbard Archipelago, Norway. The cells were aerobic, Gram-negative, rod-shaped and sometimes filamentous. Growth occurred at 4–28 °C (optimum, 20 °C), at pH 7.0–9.0 (optimum, pH 8.0) and with 0–1 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R2-35 belonged to the genus with sequence similarity to related species ranging from 91.65 to 95.19 %. Strain R2-35 contained C (10.7 %), C (9.2 %), Cω5 (16.5 %) and summed feature 3 (Cω6 and/or Cω7) (24.6 %) as the major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain R2-35 was 54.9 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R2-35 is considered to represent a novel species of the genus , for which the name sp. nov., is proposed, The type strain is R2-35 ( = CCTCC AB 2012849 = LMG 28141).

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2011CB808800)
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award 364003 and NIMR-2012-8)
  • State Oceanic Administration, PR China (Award 10/11YR06)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.061853-0
2014-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/7/2233.html?itemId=/content/journal/ijsem/10.1099/ijs.0.061853-0&mimeType=html&fmt=ahah

References

  1. Baik K. S., Kim M. S., Park S. C., Lee D. W., Lee S. D., Ka J. O., Choi S. K., Seong C. N. ( 2007 ). Spirosoma rigui sp. nov., isolated from fresh water. . Int J Syst Evol Microbiol 57, 28702873. [View Article] [PubMed]
    [Google Scholar]
  2. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. ( 1966 ). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45, 493496.[PubMed]
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  4. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [View Article] [PubMed]
    [Google Scholar]
  6. Doetsch R. N. ( 1981 ). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 2133. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  8. Finster K. W., Herbert R. A., Lomstein B. A. ( 2009 ). Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma . . Int J Syst Evol Microbiol 59, 839844. [View Article] [PubMed]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  10. Fries J., Pfeiffer S., Kuffner M., Sessitsch A. ( 2013 ). Spirosoma endophyticum sp. nov., isolated from Zn- and Cd-accumulating Salix caprea. . Int J Syst Evol Microbiol 63, 45864590. [View Article] [PubMed]
    [Google Scholar]
  11. Gosink J. J., Woese C. R., Staley J. T. ( 1998 ). Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga-Flavobacterium-Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov.. Int J Syst Bacteriol 48, 223235. [View Article] [PubMed]
    [Google Scholar]
  12. Kovacs N. ( 1956 ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178, 703. [View Article] [PubMed]
    [Google Scholar]
  13. Larkin J. M., Borrall R. ( 1984 ). Family I. Spirosomaceae Larkin and Borrall 1978, 595AL. . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 125126. Edited by Krieg N. R., Holt J. G. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  14. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297306. [View Article] [PubMed]
    [Google Scholar]
  15. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria through fatty acid analysis. . In Methods in Phytobacteriology, pp. 199204. Edited by Klement Z., Rudolph K., Sands D. C. . Budapest:: Akademiai Kaido;.
    [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  19. Ten L. N., Xu J. L., Jin F. X., Im W. T., Oh H. M., Lee S. T. ( 2009 ). Spirosoma panaciterrae sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59, 331335. [View Article] [PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  21. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  22. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  23. Xie C. H., Yokota A. ( 2003 ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49, 345349. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.061853-0
Loading
/content/journal/ijsem/10.1099/ijs.0.061853-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error