1887

Abstract

Strain JC216 was isolated from a contaminated Petri dish. Colonies were of pale yellow colour and cells were Gram-stain-negative, oxidase-positive and catalase-positive. Chitin, starch and gelatin were not hydrolysed. Strain JC216 contained Cω7/Cω6, Cω7/Cω6, C 2-OH and C as the major (≥8 %) fatty acids with minor amounts of C, C 2-OH, C 2-OH, C 2-OH, Cω6, Cω8 and Cω9. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid were the major polar lipids. Minor amounts of unidentified amino lipids and unidentified lipids were also detected. The major hopanoids identified were bacterial hopane derivatives and diplopterol. Minor amounts of diploptene and an unidentified hopanoid were also present. Spermidine was the major polyamine with minor amounts of -homospermidine and putrescine. -Acetylglucosamine and fructose were identified as major cell-wall sugars along with minor amounts of mannose and galactose. The genomic DNA G+C content was 55 mol%. Comparisons of the16S rRNA gene sequence indicated that strain JC216 represents a member of the genus in the family within the class . Strain JC216 had a sequence similarity of 97.28 % with 03SU3-P and <96.71 % with other members of the family . Furthermore, strain JC216 had 33±1 % relatedness (based on DNA–DNA hybridization) with KCTC 23340 ( = 03SU3-P). Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC216 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JC216 ( = KCTC 32445 = LMG 27671).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.061937-0
2014-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/7/2238.html?itemId=/content/journal/ijsem/10.1099/ijs.0.061937-0&mimeType=html&fmt=ahah

References

  1. Cappuccino J. G., Sherman N. ( 1999 ). Microbiology: a Laboratory Manual, 5th edn. . Menlo Park, CA:: Benjamin/Cummings;.
    [Google Scholar]
  2. Jindal S., Dua A., Lal R. ( 2013 ). Sphingopyxis indica sp. nov., isolated from a high dose point hexachlorocyclohexane (HCH)-contaminated dumpsite. . Int J Syst Evol Microbiol 63, 21862191. [View Article] [PubMed]
    [Google Scholar]
  3. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  4. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  5. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  6. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  7. Scherer P., Kneifel H. ( 1983 ). Distribution of polyamines in methanogenic bacteria. . J Bacteriol 154, 13151322.[PubMed]
    [Google Scholar]
  8. Seldin L., Dubnau D. ( 1985 ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35, 151154. [View Article]
    [Google Scholar]
  9. Shalem Raj P., Ramaprasad E. V. V., Vaseef S., Sasikala Ch., Ramana Ch. V. ( 2013 ). Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream. . Int J Syst Evol Microbiol 63, 181186. [View Article] [PubMed]
    [Google Scholar]
  10. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  11. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V. ( 2013a ). Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. . Int J Syst Evol Microbiol 63, 21322137. [View Article] [PubMed]
    [Google Scholar]
  12. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V. ( 2013b ). Vogesella alkaliphila sp. nov., isolated from an alkaline soil, and emended description of the genus Vogesella . . Int J Syst Evol Microbiol 63, 23382343. [View Article] [PubMed]
    [Google Scholar]
  13. Subhash Y., Sasikala Ch., Ramana Ch. V. ( 2013c ). Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. . Int J Syst Evol Microbiol 63, 34633469. [View Article] [PubMed]
    [Google Scholar]
  14. Takeuchi M., Hamana K., Hiraishi A. ( 2001 ). Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. . Int J Syst Evol Microbiol 51, 14051417.[PubMed]
    [Google Scholar]
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  16. Trüper H. G., Pfennig N. ( 1981 ). Isolation of members of the families Chromatiaceae and Chlorobiaceae . . In The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 279289. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  17. Venkata Ramana V., Sasikala C., Veera Venkata Ramaprasad E., Venkata Ramana C. ( 2010 ). Description of Ectothiorhodospira salini sp. nov.. J Gen Appl Microbiol 56, 313319. [View Article] [PubMed]
    [Google Scholar]
  18. Yoon J.-H., Lee C.-H., Yeo S.-H., Oh T.-K. ( 2005 ). Sphingopyxis baekryungensis sp. nov., an orange-pigmented bacterium isolated from sea water of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 55, 12231227. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.061937-0
Loading
/content/journal/ijsem/10.1099/ijs.0.061937-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error