1887

Abstract

The morphology and infraciliature of two soil ciliates, spec. nov. and Kahl, 1931, collected from Saudi Arabia, were investigated by observations of both living cells and specimens after standard staining methods. differs from its congeners by the combination of the following features: excretory pore quite near posterior end of paroral membrane, 16 or 17 somatic kineties with about 11 kinetids in each one on dorsal side, paroral membrane gently curved and composed of about 15 dikinetids, and hypostomial organelle composed of four or five files of kinetids with four monokinetids each. The diagnosis of was renewed to include characteristics revealed by the silver impregnation method: cells measuring 33–45×18–26 µm, dorsal hump and tail-like podite present, two contractile vacuoles, seven left and five right kineties, 9–11 nematodesmal rods, and dorsal brush containing about 11 basal bodies. Phylogenetic analyses based on small-subunit rRNA gene sequences showed that was closer to species of the Colpodidiidae rather than the Furgasoniidae represented by , and clustered with its congener but was a well-outlined species of the genus.

Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 31201708)
  • Deanship of Scientific Research at King Saud University
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2014-07-01
2024-04-19
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References

  1. Acosta-Mercado D., Lynn D. H. ( 2004 ). Soil ciliate species richness and abundance associated with the rhizosphere of different subtropical plant species. . J Eukaryot Microbiol 51, 582588. [View Article] [PubMed]
    [Google Scholar]
  2. Al-Farraj S. A. ( 2008 ). Ecological and taxonomic studies on the interstitial ciliates on the Red Sea coast at Obhur, Kingdom of Saudi Arabia. . Egypt J Zool 51, 7383.
    [Google Scholar]
  3. Al-Farraj S. A. ( 2011 ). First record of five soil ciliates (Ciliophora, Hypotricha) from Saudi Arabia. . Res J Biol Sci 6, 558563. [View Article]
    [Google Scholar]
  4. Al-Rasheid K. A. S. ( 1996 ). Records of free-living ciliates in Saudi Arabia. I. Marine interstitial ciliates of the Arabian Gulf islands of Al-Batinah and Abu Ali. . Arab Gulf J Sci Res 14, 747765.
    [Google Scholar]
  5. Al-Rasheid K. A. S. ( 1997 ). Records of free-living ciliates in Saudi Arabia. III. Marine interstitial ciliates of the Arabian Gulf island of Tarut. . Arab Gulf J Sci Res 15, 733766.
    [Google Scholar]
  6. Al-Rasheid K. A. S. ( 1999 ). A review of marine and brackish water interstitial ciliates from the Arabian Gulf, its offshore islands and Al-Hassa oasis with notes on their ecological status and recovery after the 1991 Gulf War oil spill. . Arab Gulf J Sci Res 17, 336368.
    [Google Scholar]
  7. Al-Rasheid K. A. S. ( 2001 ). New records of interstitial ciliates (Protozoa Ciliophora) from the Saudi coasts of the Red Sea. . Trop Zool 14, 133156. [View Article]
    [Google Scholar]
  8. Berger H., Al-Rasheid K. A. S., Foissner W. ( 2006 ). Morphology and cell division of Saudithrix terricola n. gen., n. sp., a large, stichotrich ciliate from Saudi Arabia. . J Eukaryot Microbiol 53, 260268. [View Article] [PubMed]
    [Google Scholar]
  9. Blatterer H., Foissner W. ( 1990 ). Beiträge zur ciliatenfauna (Protozoa: Ciliophora) der Amper (Bayern, Bundesrepublik Deutschland). . Arch Protistenkd 138, 93115 (in German). [View Article]
    [Google Scholar]
  10. Chao A., Li P. C., Agatha S., Foissner W. ( 2006 ). A statistical approach to estimate soil ciliate diversity and distribution based on data from five continents. . Oikos 114, 479493. [View Article]
    [Google Scholar]
  11. Chen X., Zhao Y., Al-Farraj S. A., Al-Quraishy S., El-Serehy H. A., Shao C., Al-Rasheid K. A. S. ( 2013 ). Taxonomic descriptions of two marine ciliates, Euplotes dammamensis n. sp. and Euplotes balteatus (Dujardin, 1841) Kahl, 1932 (Ciliophora, Spirotrichea, Euplotida), collected from the Arabian Gulf, Saudi Arabia. . Acta Protozool 52, 7387.
    [Google Scholar]
  12. Elwood H. J., Olsen G. J., Sogin M. L. ( 1985 ). The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata . . Mol Biol Evol 2, 399410.[PubMed]
    [Google Scholar]
  13. Fan X., Al-Farraj S. A., Gao F., Gu F. ( 2014a ). Morphological reports on two species of Dexiotricha (Ciliophora, Scuticociliatia), with a note on the phylogenetic position of the genus. . Int J Syst Evol Microbiol 64, 680688. [View Article] [PubMed]
    [Google Scholar]
  14. Fan X., Pan H., Li L., Jiang J., Al-Rasheid K. A. S., Gu F. ( 2014b ). Phylogeny of the poorly known ciliates, Microthoracida, a systematically confused taxon (Ciliophora), with morphological reports of three species. . J Eukaryot Microbiol 61, 227237. [View Article] [PubMed]
    [Google Scholar]
  15. Foissner W. ( 1987 ). Neue und wenig bekannte hypotriche und clopodide Ciliaten (Protozoa: Ciliophora) aus B öden und Moosen. . Zool Beitr 31, 187282 (in German).
    [Google Scholar]
  16. Foissner W. ( 1999 ). Notes on the soil ciliate biota (Protozoa, Ciliophora) from the Shimba Hills in Kenya (Africa): diversity and description of three new genera and ten new species. . Biodivers Conserv 8, 319389. [View Article]
    [Google Scholar]
  17. Foissner W., Adam H. ( 1981 ). Morphologie und infraciliature von Parafurgasonia sorex (Penard, 1922) nov. gen. und Obertrumia georgiana (Dragesco, 1972) nov. gen. (Protozoa: Ciliophora). . Zool Anz 207, 303319 (in German).
    [Google Scholar]
  18. Foissner W., Agatha S., Berger H. ( 2002). Soil Ciliates (Protozoa, Ciliophora) from Namibia (South West Africa), with Emphasis on Two Contrasting Environments, the Etosha Region and the Namib Desert (Denisia, vol. 5) . Linz:: Oberösterreichisches Landesmuseum;.
    [Google Scholar]
  19. Foissner W., Quintela-Alonso P., Al-Rasheid K. ( 2008 ). Soil ciliates from Saudi Arabia, including descriptions of two new genera and six new species. . Acta Protozool 47, 317352.[PubMed]
    [Google Scholar]
  20. Gao S., Huang J., Li J., Song W. ( 2012 ). Molecular phylogeny of the cyrtophorid ciliates (Protozoa, Ciliophora, Phyllopharyngea). . PLoS ONE 7, e33198. [View Article] [PubMed]
    [Google Scholar]
  21. Gong J., Kim S. J., Kim S. Y., Min G. S., Roberts D. M., Warren A., Choi J. K. ( 2007 ). Taxonomic redescriptions of two ciliates, Protogastrostyla pulchra n. g., n. comb. and Hemigastrostyla enigmatica (Ciliophora: Spirotrichea, Stichotrichia), with phylogenetic analyses based on 18S and 28S rRNA gene sequences. . J Eukaryot Microbiol 54, 468478.[PubMed]
    [Google Scholar]
  22. Gong J., Stoeck T., Yi Z., Miao M., Zhang Q., Roberts D. M., Warren A., Song W. ( 2009 ). Small subunit rRNA phylogenies show that the class Nassophorea is not monophyletic (Phylum Ciliophora). . J Eukaryot Microbiol 56, 339347. [View Article] [PubMed]
    [Google Scholar]
  23. He L., Zeng H., Shen J., Niu Y., Lou H., Gu F. ( 2006 ). An improved FLUTAX method for visualizing the microtubule organelle of ciliates. . Chin J Zool 41, 5661.
    [Google Scholar]
  24. Jankowski A. W. ( 2007 ). Phylum Ciliophora Doflein, 1901. Review of taxa. . In Protista Part 2: Handbook on Zoology, pp. 415993. Edited by Alimov A. F. . St Petersburg:: Nauka (in Russian with English summary);.
    [Google Scholar]
  25. Kahl A. ( 1931 ). Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria). 2. Holotricha. . In Die Tierwelt Deutschlands, vol. 21, pp. 181398. Edited by Dahl F. . Jena:: Gustav Fischer;. (in German)
    [Google Scholar]
  26. Lynn D. H. ( 2008 ). In The Ciliated Protozoa, Characterization, Classification, and Guide to the Literature, , 3rd edn., pp. 414416. Dordrecht:: Springer;.
    [Google Scholar]
  27. Ma H., Choi J. K., Song W. ( 2003 ). An improved silver carbonate impregnation for marine ciliated protozoa. . Acta Protozool 42, 161164.
    [Google Scholar]
  28. Medlin L., Elwood H. J., Stickel S., Sogin M. L. ( 1988 ). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. . Gene 71, 491499. [View Article] [PubMed]
    [Google Scholar]
  29. Miller M. A., Pfeiffer W., Schwartz T. ( 2010 ). Creating the CIPRES Science Gateway for inference of large phylogenetic trees. . In Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, LA, USA, 14 November 2010 , pp. 18. New York:: IEEE;. [CrossRef]
    [Google Scholar]
  30. Nylander J. A. A. ( 2004 ). MrModeltest v2. Uppsala:: Evolutionary Biology Centre, Uppsala University;.
    [Google Scholar]
  31. Omar A., Foissner W. ( 2012 ). Neotypification and ontogenesis of Leptopharynx costatus costatus Mermod, 1914. . J Eukaryot Microbiol 59, 268286. [View Article] [PubMed]
    [Google Scholar]
  32. Pan H., Lin X., Gong J., Al-Rasheid K. A. S., Song W. ( 2012 ). Taxonomy of five species of cyrtophorids (Protozoa: Ciliophora) including consideration of the phylogeny of two new genera. . Zool J Linn Soc 164, 117. [View Article]
    [Google Scholar]
  33. Penn O., Privman E., Ashkenazy H., Landan G., Graur D., Pupko T. ( 2010a ). guidance: a web server for assessing alignment confidence scores. . Nucleic Acids Res 38 (Web Server), W23W28. [View Article] [PubMed]
    [Google Scholar]
  34. Penn O., Privman E., Landan G., Graur D., Pupko T. ( 2010b ). An alignment confidence score capturing robustness to guide tree uncertainty. . Mol Biol Evol 27, 17591767. [View Article] [PubMed]
    [Google Scholar]
  35. Posada D., Crandall K. A. ( 1998 ). modeltest: testing the model of DNA substitution. . Bioinformatics 14, 817818. [View Article] [PubMed]
    [Google Scholar]
  36. Ronquist F., Huelsenbeck J. P. ( 2003 ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19, 15721574. [View Article] [PubMed]
    [Google Scholar]
  37. Stamatakis A., Hoover P., Rougemont J. ( 2008 ). A rapid bootstrap algorithm for the RAxML Web servers. . Syst Biol 57, 758771. [View Article] [PubMed]
    [Google Scholar]
  38. Verni F., Rosati G. ( 2011 ). Resting cysts: a survival strategy in Protozoa Ciliophora. . Ital J Zool 78, 134145. [View Article]
    [Google Scholar]
  39. Wilbert N. ( 1975 ). Eine verbesserte Technik der Protargolimprägnation für Ciliaten. . Mikrokosmos 64, 171179 (in German).
    [Google Scholar]
  40. Yan Y., Xu Y., Yi Z., Warren A. ( 2013 ). Redescriptions of three trachelocercid ciliates (Protista, Ciliophora, Karyorelictea), with notes on their phylogeny based on small subunit rRNA gene sequences. . Int J Syst Evol Microbiol 63, 35063514. [View Article] [PubMed]
    [Google Scholar]
  41. Zhang Q., Yi Z., Fan X., Warren A., Gong J., Song W. ( 2014 ). Further insights into the phylogeny of two ciliate classes Nassophorea and Prostomatea (Protista, Ciliophora). . Mol Phylogenet Evol 70,162170. [View Article] [PubMed]
    [Google Scholar]
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