RT Journal Article SR Electronic(1) A1 Jiang, Zhao A1 Zhang, Dao-Feng A1 Khieu, Thi-Nhan A1 Son, Chu Ky A1 Zhang, Xiao-Mei A1 Cheng, Juan A1 Tian, Xin-Peng A1 Zhang, Si A1 Li, Wen-JunYR 2014 T1 Bacillus tianshenii sp. nov., isolated from a marine sediment sample JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_6 SP 1998 OP 2002 DO https://doi.org/10.1099/ijs.0.062224-0 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-positive, motile, catalase- and oxidase-positive, aerobic, endospore-forming, peritrichous, rod-shaped bacterium, designated YIM M13235T, was isolated from a marine sediment sample collected from the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M13235T belonged to the genus Bacillus . The strain grew optimally at 30 °C, pH 7.0 and in the presence of 2–4 % (w/v) NaCl. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. Strain YIM M13235T exhibited a menaquinone system with MK-7, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unknown phospholipids and one unknown glycolipid. The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, iso-C17 : 1ω10c and summed feature 4 (anteiso-C17 : 1 and/or iso-C17 : 1). The genomic DNA G+C content was 42.1 mol%. The DNA–DNA relatedness values between strain YIM M13235T and its close relatives (16S rRNA gene sequence similarities >97 %) including Bacillus halmapalus DSM 8723T, Bacillus horikoshii DSM 8719T and Bacillus zhanjiangensis JSM 099021T were 41 %, 44 % and 44 %, respectively. On the basis of genotypic, phenotypic and DNA–DNA relatedness data, it is apparent that strain YIM M13235T represents a novel species of the genus Bacillus , for which the name Bacillus tianshenii sp. nov. is proposed. The type strain is YIM M13235T ( = DSM 25879T = KCTC 33044T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.062224-0