1887

Abstract

We define two novel species of the genus that are phenotypically similar to and have near identical 16S rRNA gene sequences to . However, compared to and each other, the two species, sp. nov. (type strain MSHR1132 = DSM 28299 = SSI 89.005) and sp. nov. (type strain FSA084 = DSM 28300 = SSI 89.004), demonstrate: 1) at a whole-genome level considerable phylogenetic distance, lack of admixture, average nucleotide identity <95 %, and inferred DNA–DNA hybridization <70 %; 2) different profiles as determined by MALDI-TOF MS; 3) a non-pigmented phenotype for sp. nov.; 4) sp. nov. is not detected by standard PCR; 5) distinct peptidoglycan types compared to ; 6) a separate ecological niche for sp. nov.; and 7) a distinct clinical disease profile for sp. nov. compared to .

Funding
This study was supported by the:
  • Wellcome Trust
  • Deutsche Forschungsgemeinschaft (Award EI 247/8-1)
  • Australian National Health and Medical Research Council Career Development Fellow (Award 1065736)
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062752-0
2015-01-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/1/15.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062752-0&mimeType=html&fmt=ahah

References

  1. Akobi B., Aboderin O., Sasaki T., Shittu A. ( 2012 ). Characterization of Staphylococcus aureus isolates from faecal samples of the Straw-Coloured Fruit Bat (Eidolon helvum) in Obafemi Awolowo University (OAU), Nigeria. . BMC Microbiol 12, 279. [View Article] [PubMed]
    [Google Scholar]
  2. Brakstad O. G., Aasbakk K., Maeland J. A. ( 1992 ). Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. . J Clin Microbiol 30, 16541660.[PubMed]
    [Google Scholar]
  3. Brennan L., Lilliebridge R. A., Cheng A. C., Giffard P. M., Currie B. J., Tong S. Y. ( 2013 ). Community-associated meticillin-resistant Staphylococcus aureus carriage in hospitalized patients in tropical northern Australia. . J Hosp Infect 83, 205211. [View Article] [PubMed]
    [Google Scholar]
  4. Cheng L., Connor T. R., Sirén J., Aanensen D. M., Corander J. ( 2013 ). Hierarchical and spatially explicit clustering of DNA sequences with BAPS software. . Mol Biol Evol 30, 12241228. [View Article] [PubMed]
    [Google Scholar]
  5. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007 ). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. . Int J Syst Evol Microbiol 57, 8191. [View Article] [PubMed]
    [Google Scholar]
  6. Gotz F., Bannerman T., Schleifer K.-H. ( 2006 ). The genera Staphylococcus and Macrococcus . . In The Prokaryotes, 3rd edn, Vol. 4 Bacteria: Firmicutes, Cyanobacteria, pp. 5–75. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York, NY:: Springer;. [View Article]
    [Google Scholar]
  7. Holt D. C., Holden M. T., Tong S. Y., Castillo-Ramirez S., Clarke L., Quail M. A., Currie B. J., Parkhill J., Bentley S. D. et al. ( 2011 ). A very early-branching Staphylococcus aureus lineage lacking the carotenoid pigment staphyloxanthin. . Genome Biol Evol 3, 881895. [View Article] [PubMed]
    [Google Scholar]
  8. Jenney A., Holt D., Ritika R., Southwell P., Pravin S., Buadromo E., Carapetis J., Tong S., Steer A. ( 2014 ). The clinical and molecular epidemiology of Staphylococcus aureus infections in Fiji. . BMC Infect Dis 14, 160. [View Article] [PubMed]
    [Google Scholar]
  9. Kloos W. E., Schleifer K. H., Smith R. F. ( 1976 ). Characterization of Staphylococcus sciuri sp. nov. and its subspecies. . Int J Syst Bacteriol 26, 2237. [View Article]
    [Google Scholar]
  10. McDonald M., Dougall A., Holt D., Huygens F., Oppedisano F., Giffard P. M., Inman-Bamber J., Stephens A. J., Towers R. et al. ( 2006 ). Use of a single-nucleotide polymorphism genotyping system to demonstrate the unique epidemiology of methicillin-resistant Staphylococcus aureus in remote aboriginal communities. . J Clin Microbiol 44, 37203727. [View Article] [PubMed]
    [Google Scholar]
  11. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M. ( 2013 ). Genome sequence-based species delimitation with confidence intervals and improved distance functions. . BMC Bioinformatics 14, 60. [View Article] [PubMed]
    [Google Scholar]
  12. Ng J. W., Holt D. C., Lilliebridge R. A., Stephens A. J., Huygens F., Tong S. Y., Currie B. J., Giffard P. M. ( 2009 ). Phylogenetically distinct Staphylococcus aureus lineage prevalent among indigenous communities in northern Australia. . J Clin Microbiol 47, 22952300. [View Article] [PubMed]
    [Google Scholar]
  13. Richter M., Rosselló-Móra R. ( 2009 ). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106, 1912619131. [View Article] [PubMed]
    [Google Scholar]
  14. Ruimy R., Angebault C., Djossou F., Dupont C., Epelboin L., Jarraud S., Lefevre L. A., Bes M., Lixandru B. E. et al. ( 2010 ). Are host genetics the predominant determinant of persistent nasal Staphylococcus aureus carriage in humans?. J Infect Dis 202, 924934. [View Article] [PubMed]
    [Google Scholar]
  15. Schaumburg F., Alabi A. S., Köck R., Mellmann A., Kremsner P. G., Boesch C., Becker K., Leendertz F. H., Peters G. ( 2012 ). Highly divergent Staphylococcus aureus isolates from African non-human primates. . Environ Microbiol Rep 4, 141146. [View Article] [PubMed]
    [Google Scholar]
  16. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  17. Schleifer K. H., Geyer U., Kilpper-Bälz R., Devriese L. A. ( 1983 ). Elevation of Staphylococcus sciuri subsp. lentus (Kloos et al.) to species status: Staphylococcus lentus (Kloos et al.) comb. nov.. Syst Appl Microbiol 4, 382387. [View Article] [PubMed]
    [Google Scholar]
  18. Schumann P. ( 2011 ). Peptidoglycan Structure. . Methods Microbiol 38, 101129.. [View Article]
    [Google Scholar]
  19. Švec P., Vancanneyt M., Sedláček I., Engelbeen K., Štětina V., Swings J., Petráš P. ( 2004 ). Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwińska et al. 1995 as a later synonym of Staphylococcus vitulinus Webster et al. 1994. . Int J Syst Evol Microbiol 54, 22132215. [View Article] [PubMed]
    [Google Scholar]
  20. Tong S. Y., Lilliebridge R. A., Bishop E. J., Cheng A. C., Holt D. C., McDonald M. I., Giffard P. M., Currie B. J., Boutlis C. S. ( 2010 ). Clinical correlates of Panton-Valentine leukocidin (PVL), PVL isoforms, and clonal complex in the Staphylococcus aureus population of Northern Australia. . J Infect Dis 202, 760769. [View Article] [PubMed]
    [Google Scholar]
  21. Tong S. Y., Sharma-Kuinkel B. K., Thaden J. T., Whitney A. R., Yang S. J., Mishra N. N., Rude T., Lilliebridge R. A., Selim M. A. et al. ( 2013 ). Virulence of endemic nonpigmented northern Australian Staphylococcus aureus clone (clonal complex 75, S. argenteus) is not augmented by staphyloxanthin. . J Infect Dis 208, 520527. [View Article] [PubMed]
    [Google Scholar]
  22. Zakrzewska-Czerwińska J., Gaszewska-Mastalarz A., Lis B., Gamian A., Mordarski M. ( 1995 ). Staphylococcus pulvereri sp. nov., isolated from human and animal specimens. . Int J Syst Bacteriol 45, 169172. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062752-0
Loading
/content/journal/ijsem/10.1099/ijs.0.062752-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error