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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_2

gen. nov., sp. nov., a member of the family , isolated from sludge compost Free

Abstract

Two novel Gram-staining-positive, rod-shaped, endospore-forming and moderately thermophilic bacteria, designated strains DX-3 and GIESS002, were isolated from sludge composts from Guangdong Province, China. Analysis of 16S rRNA gene sequences revealed that the isolates were closely related to each other with extremely high similarity (99.6 %), and were members of the family . However, these two isolates formed a novel phylogenetic branch within this family. Their closest relatives were the members of the genera , and . Cells of both strains were facultatively anaerobic and catalase- and oxidase-positive. The cell-wall peptidoglycan type was A1γ (-diaminopimelic acid direct). The predominant isoprenoid quinone was MK-7. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acid was iso-C. The DNA G+C content was 43.2–43.7 mol%. The results of a polyphasic taxonomic study indicated that strains DX-3 and GIESS002 represent a novel species in a new genus in the family , order , for which the name gen. nov., sp. nov. is proposed. The type strain is DX-3 ( = KCTC 33104 = CGMCC 1.12360).

  • Published Online:
Funding
This study was supported by the:
  • Agricultural Science & Technology Achievements Transfer Project of Guangdong Province, China (Award 2012NL040)
  • National Natural Science Foundation of China (Award 31100353)
  • Foundation for Excellent Young Scientist in Guangdong Academy of Sciences (Award qnjj201401)
© 2015 IUMS
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2015-02-01
2024-04-19
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Compostibacillus humi gen. nov., sp. nov., a member of the family Bacillaceae, isolated from sludge compost
Int J Syst Evol Microbiol 65, 346 (2015); https://doi.org/10.1099/ijs.0.062869-0
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