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Volume 65, Issue Pt_1

sp. nov., isolated from a termite gut Free

Abstract

Strain TS-56 was isolated from the gut of a wood-feeding termite, Snyder. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strain represented a member of the genus of the family , with sequence similarities to other species of the genus ranging from 96.6 % to 97.8 %. The isolate was Gram-stain-positive, non-motile, with light yellow colonies and irregular short rod-shaped cells (0.4–0.6 µm in diameter, 0.6–1.0 µm in length). Growth of TS-56 occurred at 20–35 °C (optimum, 30 °C) and at pH 4.0–8.0 (optimum, pH 5.0). The peptidoglycan of TS-56 contained ornithine, glutamic acid, alanine, homoserine and glycine. The acyl type was acetyl. The most abundant cellular fatty acid of TS-56 was cyclohexyl-C (88.79 %). The respiratory menaquinone was MK-11. The polar lipid profile contained disphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown glycolipids. DNA of the type strain had a G+C content of 67.4 mol%. On the basis of the phylogenetic properties and phenotypic distinctiveness, TS-56 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TS-56 ( = CGMCC 1.10363 = NBRC 109838).

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970001, SKLMR-20110605 and 31170004)
  • State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
© 2015 School of Life Sciences, Central China Normal University, Wuhan, China
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2015-01-01
2024-04-20
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403–410. [View Article] [PubMed]
    [Google Scholar]
  2. Behrendt U., Ulrich A., Schumann P., Naumann D., Suzuki K. ( 2002 ). Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov.. Int J Syst Evol Microbiol 52, 1441–1454. [View Article] [PubMed]
    [Google Scholar]
  3. Chen W., Wang B., Hong H., Yang H., Liu S.-J. ( 2012 ). Deinococcus reticulitermitis sp. nov., isolated from a termite gut. . Int J Syst Evol Microbiol 62, 78–83. [View Article] [PubMed]
    [Google Scholar]
  4. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  5. Dong X.-Z., Cai M.-Y. (editors) ( 2001 ). Determination of biochemical properties. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing:: Science Press; (in Chinese).
     [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  8. Kim M. K., Kim Y. J., Kim H. B., Kim S. Y., Yi T. H., Yang D. C. ( 2008 ). Curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 58, 2393–2397. [View Article] [PubMed]
    [Google Scholar]
  9. Kim H., Park D.-S., Oh H.-W., Lee K. H., Chung D.-H., Park H.-Y., Park H.-M., Bae K. S. ( 2012a ). Gryllotalpicola gen. nov., with descriptions of Gryllotalpicola koreensis sp. nov., Gryllotalpicola daejeonensis sp. nov. and Gryllotalpicola kribbensis sp. nov. from the gut of the African mole cricket, Gryllotalpa africana, and reclassification of Curtobacterium ginsengisoli as Gryllotalpicola ginsengisoli comb. nov.. Int J Syst Evol Microbiol 62, 2363–2370. [View Article] [PubMed]
    [Google Scholar]
  10. Kim B.-C., Park D.-S., Kim H., Oh H.-W., Lee K. H., Shin K.-S., Bae K. S. ( 2012b ). Herbiconiux moechotypicola sp. nov., a xylanolytic bacterium isolated from the gut of hairy long-horned toad beetles, Moechotypa diphysis (Pascoe). . Int J Syst Evol Microbiol 62, 90–95. [View Article] [PubMed]
    [Google Scholar]
  11. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109–118. [View Article] [PubMed]
    [Google Scholar]
  12. Park Y. H., Suzuki K., Yim D. G., Lee K. C., Kim E., Yoon J., Kim S., Kho Y. H., Goodfellow M., Komagata K. ( 1993 ). Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. . Antonie van Leeuwenhoek 64, 307–313. [View Article] [PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  14. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226–231.[PubMed]
    [Google Scholar]
  15. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 1596–1599. [View Article] [PubMed]
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  17. Uchida K., Kudo T., Suzuki K.-I., Nakase T. ( 1999 ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. . J Gen Appl Microbiol 45, 49–56. [View Article] [PubMed]
    [Google Scholar]
  18. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989). Analysis of menaquinone compound in microbial cells by HPLC.. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178. [View Article]
    [Google Scholar]
  19. Yamada K., Komagata K. ( 1972 ). Taxonomic studies on coryneform bacteria. V. Classification of coryneform bacteria. . J Gen Appl Microbiol 18, 417–431. [View Article]
    [Google Scholar]
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Gryllotalpicola reticulitermitis sp. nov., isolated from a termite gut
Int J Syst Evol Microbiol 65, 85 (2015); https://doi.org/10.1099/ijs.0.062984-0
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