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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_10

Description of sp. nov., an endophytic actinobacterium isolated from Aloe vera Free

Abstract

A yellow Gram-stain-positive, non-motile, non-endospore -forming, spherical endophytic actinobacterium, designated strain AE-6, was isolated from the inner fleshy leaf tissues of (Aloe vera) collected from Pune, Maharashtra, India. Strain AE-6 grew at high salt concentrations [10 % (w/v) NaCl], temperatures of 15–41 °C and a pH range of 5–12. It showed highest (99.7 %) 16S rRNA gene sequence similarity with YIM 65004 followed by NCTC 2665 (99.6 %) and YIM 56238 (99.0 %). Ribosomal protein profiling by MALDI-TOF/MS also showed it was most closely related to YIM 65004 and NCTC 2665. Like other members of the genus , strain AE-6 had a high content of branched chain fatty acids (iso-C15 : 0 and anteiso-C15 : 0). MK-8(H) and MK-8 were the predominant isoprenoid quinones. Cell wall analysis showed an ‘A2 -Lys-peptide subunit’ type of peptidoglycan and ribose to be the major cell wall sugar. The DNA G+C content was 70 mol%. Results of DNA–DNA hybridization of AE-6 with its closest relatives from the genus produced a value of less than 70%. Based on the results of this study, strain AE-6 could be clearly differentiated from other members of the genus We propose that it represents a novel species of the genus and suggest the name sp. nov., with strain AE-6 ( = MCC 2184 = DSM 27472) as the type strain of the species.

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Funding
This study was supported by the:
  • Department of Biotechnology (Award BT/PR/0054/NDB/52/94/2007)
© 2014 IUMS
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2014-10-01
2024-04-25
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Description of Micrococcusaloeverae sp. nov., an endophytic actinobacterium isolated from Aloe vera
Int J Syst Evol Microbiol 64, 3427 (2014); https://doi.org/10.1099/ijs.0.063339-0
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