Description of Micrococcusaloeverae sp. nov., an endophytic actinobacterium isolated from Aloe vera
Prakash, Om and Nimonkar, Yogesh and Munot, Hitendra and Sharma, Avinash and Vemuluri, Venkata Ramana and Chavadar, Mahesh S. and Shouche, Yogesh S.,, 64, 3427-3433 (2014),
doi = https://doi.org/10.1099/ijs.0.063339-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A yellow Gram-stain-positive, non-motile, non-endospore -forming, spherical endophytic actinobacterium, designated strain AE-6T, was isolated from the inner fleshy leaf tissues of Aloe barbadensis (Aloe vera) collected from Pune, Maharashtra, India. Strain AE-6T grew at high salt concentrations [10 % (w/v) NaCl], temperatures of 15–41 °C and a pH range of 5–12. It showed highest (99.7 %) 16S rRNA gene sequence similarity with Micrococcus yunnanensis YIM 65004T followed by Micrococcus luteus NCTC 2665T (99.6 %) and Micrococcus endophyticus YIM 56238T (99.0 %). Ribosomal protein profiling by MALDI-TOF/MS also showed it was most closely related to M. yunnanensis YIM 65004T and M. luteus NCTC 2665T. Like other members of the genus Micrococcus , strain AE-6T had a high content of branched chain fatty acids (iso-C15 : 0 and anteiso-C15 : 0). MK-8(H2) and MK-8 were the predominant isoprenoid quinones. Cell wall analysis showed an ‘A2 l-Lys-peptide subunit’ type of peptidoglycan and ribose to be the major cell wall sugar. The DNA G+C content was 70 mol%. Results of DNA–DNA hybridization of AE-6T with its closest relatives from the genus Micrococcus produced a value of less than 70%. Based on the results of this study, strain AE-6T could be clearly differentiated from other members of the genus Micrococcus . We propose that it represents a novel species of the genus Micrococcus and suggest the name Micrococcus aloeverae sp. nov., with strain AE-6T ( = MCC 2184T = DSM 27472T) as the type strain of the species.,
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