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Volume 64, Issue Pt_9

gen. nov., sp. nov., a mesophilic bacterium isolated from surface seawater and description of fam. nov. in the class Free

Abstract

A Gram-stain-negative, motile, mesophilic, aerobic, rod-shaped bacterium, strain 5-11, was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. The strain exhibited a narrow growth temperature range of 20–30 °C. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the order in the class and was related most closely to the genus (up to 91.2 % similarity to the type strains of species of the genus) but branched deeply from species of . The major fatty acids were iso-Cω9, iso-C, and Cω7 and/or iso-C 2-OH. Ubiquinone-10 (Q-10) was detected as the sole isoprenoid quinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. Although strains of have been shown to contain unidentified glycolipids, they were not detected from strain 5-11. The DNA G+C content of strain 5-11 was 44.3 mol%, a value that was lower than those of strains of (50–58 mol%) and was relatively low for the members of the class . On the basis of phenotypic, genotypic and chemotaxonomic data, it is proposed that strain 5-11 represents a novel species of a new genus, gen. nov., sp. nov., within a new family fam. nov. The type strain of is 5-11 ( = NBRC 110045 = LMG 28278).

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Funding
This study was supported by the:
  • Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
© 2014 IUMS
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2014-09-01
2024-04-20
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Temperatibacter marinus gen. nov., sp. nov., a mesophilic bacterium isolated from surface seawater and description of Temperatibacteraceae fam. nov. in the class Alphaproteobacteria
Int J Syst Evol Microbiol 64, 3075 (2014); https://doi.org/10.1099/ijs.0.063685-0
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