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Volume 64, Issue Pt_10

Description of sp. nov., isolated from a methanogenic reactor of cattle waste, reclassification of and as comb. nov. and comb. nov. and emended description of the genus Free

Abstract

A strictly anaerobic bacterial strain, WK011, was isolated from a methanogenic reactor treating waste from cattle farms. The cells stained Gram-negative and were curved rods with a polar or subpolar flagellum. Spore formation was not observed. The optimum temperature for growth was 35 °C and the optimum pH was 6.7. Tests for oxidase, catalase and nitrate-reduction activities were negative. Hydrogen sulfide was produced. The strain fermented carbohydrates and produced acetate and propionate as major fermentation products. The genomic DNA G+C content was 41.7 mol%. The major cellular fatty acids were C, Cω9 and C dimethylacetal. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. The most closely related species to strain WK011 on the basis of 16S rRNA gene sequences were and (95.6 % sequence similarity to the type strains of both species). It was shown by phylogenetic and phenotypic examination of the type strains of related species, including the second species of the genus , , that the two genera should be combined and that the two species of the genus should be transferred to the genus as comb. nov. (type strain SH2 = ATCC 49691 = DSM 20765) and comb. nov. (type strain AA1 = ATCC 49689 = DSM 20756), with an emended description of the genus . Based on differences in the phylogenetic and phenotypic characteristics of strain WK011 from those of closely related species, the novel species sp. nov. is proposed to accommodate the strain. The type strain is WK011 ( = JCM 16475 = DSM 22929).

  • Published Online:
Funding
This study was supported by the:
  • Institute for Fermentation, Osaka
IUMS
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2014-10-01
2024-04-16
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Description of Propionispira arcuata sp. nov., isolated from a methanogenic reactor of cattle waste, reclassification of Zymophilus raffinosivorans and Zymophilus paucivorans as Propionispiraraffinosivorans comb. nov. and Propionispira paucivorans comb. nov. and emended description of the genus Propionispira
Int J Syst Evol Microbiol 64, 3571 (2014); https://doi.org/10.1099/ijs.0.063875-0
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