1887

Abstract

Two strains were isolated from oral cavity samples of healthy elephants. The isolates were Gram-positive, catalase-negative, coccus-shaped organisms that were tentatively identified as a streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene sequence analysis suggested classification of these organisms in the genus with ATCC 19642 and NUM 1001 as their closest phylogenetic neighbours with 98.2 and 96.9 % gene sequence similarity, respectively. When multi-locus sequence analysis using four housekeeping genes, and , was carried out, similarity of concatenated sequences of the four housekeeping genes from the new isolates and was 89.7 %. DNA–DNA hybridization experiments suggested that the new isolates were distinct from and other species of the genus . On the basis of genotypic and phenotypic differences, it is proposed that the novel isolates are classified in the genus as representatives of sp. nov. The type strain of is NUM 2101 ( = JCM 19285 = DSM 27377).

Funding
This study was supported by the:
  • Nihon University School of Dentistry at Matsudo
  • Japan Society for the Promotion of Science for Young Scientists (Award B:25870786)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.064048-0
2014-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/11/3755.html?itemId=/content/journal/ijsem/10.1099/ijs.0.064048-0&mimeType=html&fmt=ahah

References

  1. Beighton D., Russell R. R. B., Hayday H. ( 1981 ). The isolation and characterization of Streptococcus mutans serotype h from dental plaque of monkeys (Macaca fascicularis). . J Gen Microbiol 124, 271279.[PubMed]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  3. Facklam R. ( 2002 ). What happened to the streptococci: overview of taxonomic and nomenclature changes. . Clin Microbiol Rev 15, 613630. [View Article] [PubMed]
    [Google Scholar]
  4. Glazunova O. O., Raoult D., Roux V. ( 2009 ). Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus . . Int J Syst Evol Microbiol 59, 23172322. [View Article] [PubMed]
    [Google Scholar]
  5. Hamada S., Slade H. D. ( 1980 ). Biology, immunology, and cariogenicity of Streptococcus mutans . . Microbiol Rev 44, 331384.[PubMed]
    [Google Scholar]
  6. Hiraishi A. ( 1992 ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. . Lett Appl Microbiol 15, 210213. [View Article] [PubMed]
    [Google Scholar]
  7. Hirasawa M., Takada K. ( 1994 ). Porphyromonas gingivicanis sp. nov. and Porphyromonas crevioricanis sp. nov., isolated from beagles. . Int J Syst Bacteriol 44, 637640. [View Article] [PubMed]
    [Google Scholar]
  8. Hirasawa M., Kiyono H., Shiota T., Michalek S. M., McGhee J. R. ( 1980 ). Virulence of Streptococcus mutans: immunochemical characterization of a serotype g-defective mutant (C307). . Infect Immun 27, 697699.[PubMed]
    [Google Scholar]
  9. Huch M., De Bruyne K., Cleenwerck I., Bub A., Cho G. S., Watzl B., Snauwaert I., Franz C. M. A. P., Vandamme P. ( 2013 ). Streptococcus rubneri sp. nov., isolated from the human throat. . Int J Syst Evol Microbiol 63, 40264032. [View Article] [PubMed]
    [Google Scholar]
  10. Johnson J. L. ( 1973 ). Use of nucleic-acid homologies in the taxonomy of anaerobic bacteria. . Int J Syst Bacteriol 23, 308315. [View Article]
    [Google Scholar]
  11. Kadri Z., Amar M., Ouadghiri M., Cnockaert M., Aerts M., El Farricha O., Vandamme P. ( 2014 ). Streptococcus moroccensis sp. nov. and Streptococcus rifensis sp. nov., isolated from raw camel milk. . Int J Syst Evol Microbiol 64, 24802485. [View Article] [PubMed]
    [Google Scholar]
  12. Nakano K., Nomura R., Nakagawa I., Hamada S., Ooshima T. ( 2004 ). Demonstration of Streptococcus mutans with a cell wall polysaccharide specific to a new serotype, k, in the human oral cavity. . J Clin Microbiol 42, 198202. [View Article] [PubMed]
    [Google Scholar]
  13. Okamoto M., Imai S., Miyanohara M., Saito W., Momoi Y., Abo T., Nomura Y., Ikawa T., Ogawa T. & other authors ( 2013 ). Streptococcus troglodytae sp. nov., from the chimpanzee oral cavity. . Int J Syst Evol Microbiol 63, 418422. [View Article] [PubMed]
    [Google Scholar]
  14. Rantz L. A., Randall E. ( 1955 ). Use of autoclaved extracts of hemolytic streptococci for serological grouping. . Stanford Med Bull 13, 290291.[PubMed]
    [Google Scholar]
  15. Shinozaki-Kuwahara N., Takada K., Hirasawa M. ( 2011 ). Streptococcus ursoris sp. nov., isolated from the oral cavities of bears. . Int J Syst Evol Microbiol 61, 4044. [View Article] [PubMed]
    [Google Scholar]
  16. Shiroza T., Shinozaki N., Watanabe T., Ikemi T., Fukushima K., Abiko Y. ( 1998 ). Rapid isolation of chromosomal DNA from oral streptococci and polymerase chain reaction-oriented restriction fragment-length polymorphism analysis for genetic heterogeneity. . Oral Microbiol Immunol 13, 1116. [View Article] [PubMed]
    [Google Scholar]
  17. Tabchoury C. P. M., Sousa M. C. K., Arthur R. A., Mattos-Graner R. O., Del Bel Cury A. A., Cury J. A. ( 2008 ). Evaluation of genotypic diversity of Streptococcus mutans using distinct arbitrary primers. . J Appl Oral Sci 16, 403407. [View Article] [PubMed]
    [Google Scholar]
  18. Takada K., Hirasawa M. ( 2007 ). Streptococcus orisuis sp. nov., isolated from the pig oral cavity. . Int J Syst Evol Microbiol 57, 12721275. [View Article] [PubMed]
    [Google Scholar]
  19. Takada K., Hirasawa M. ( 2008 ). Streptococcus dentirousetti sp. nov., isolated from the oral cavities of bats. . Int J Syst Evol Microbiol 58, 160163. [View Article] [PubMed]
    [Google Scholar]
  20. Takada K., Wyszomirska J., Shiota T. ( 1984 ). Serological characterization of Streptococcus mutans serotype polysaccharide g and its different molecular weight forms. . Infect Immun 45, 464469.[PubMed]
    [Google Scholar]
  21. Takada K., Igarashi M., Yamaguchi Y., Hirasawa M. ( 2008 ). New serotype of mutans streptococci isolated from pig oral cavity. . Microbiol Immunol 52, 6468. [View Article] [PubMed]
    [Google Scholar]
  22. Takada K., Hayashi K., Sato Y., Hirasawa M. ( 2010 ). Streptococcus dentapri sp. nov., isolated from the wild boar oral cavity. . Int J Syst Evol Microbiol 60, 820823. [View Article] [PubMed]
    [Google Scholar]
  23. Takada K., Saito M., Tsudukibashi O., Hiroi T., Hirasawa M. ( 2013 ). Streptococcus orisasini sp. nov. and Streptococcus dentasini sp. nov., isolated from the oral cavity of donkeys. . Int J Syst Evol Microbiol 63, 27822786. [View Article] [PubMed]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  25. Whiley R. A., Beighton D. ( 1998 ). Current classification of the oral streptococci. . Oral Microbiol Immunol 13, 195216. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.064048-0
Loading
/content/journal/ijsem/10.1099/ijs.0.064048-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error