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Volume 64, Issue Pt_9

sp. nov., a methyl parathion degrading bacterium, isolated from methyl parathion contaminated soil Free

Abstract

A methyl parathion (MP) degrading bacterial strain, designated MP-1, was isolated from a waste land where pesticides were formerly manufactured in Jiangsu province, China. Polyphasic taxonomic studies showed that MP-1 is a Gram-stain-negative, non-spore-forming, rod-shaped and motile bacterium. The bacterium could grow at salinities of 0–1 % (w/v) and temperatures of 15–40 °C. Strain MP-1 could reduce nitrate to nitrite, utilize -glucose and -arabinose, but not produce indole, or hydrolyse gelatin. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that MP-1 belongs to the genus , showing highest sequence similarity to DSM 25160 (98.5 %), and similar strains including OP-1 (98.2 %), LMG 22940 (97.5 %), DSM 50014 (97.4 %), LMG 22937 (97.2 %) and LMG 22936 (97.0 %). In addition, the and gene segments of strain MP-1 exhibited less than 89.0 % and 95.1 % similarities with the most highly-related type strains indicated above. The G+C content of strain MP-1 was 62.6 mol%. The major isoprenoid quinone was ubiquinone Q-8. The predominant polar lipids comprised phosphatidyl ethanolamine, phosphatidyl glycerol, aminolipid and phospholipid. The principal fatty acids in strain MP-1 were Cω7/Cω6 (23.3 %), C (16.8 %), cyclo-C (15.0 %), Cω7/Cω6 (8.5 %), cyclo-Cω8 (8.1 %), C iso I/C 3-OH (5.7 %), C 3-OH (5.6 %) and C2-OH (5.1 %). The DNA–DNA relatedness values between strain MP-1 and the three type strains ( DSM 25160, OP-1 and DSM 50014) ranged from 24.6 % to 37.4 %. In accordance with phenotypic and genotypic characteristics, strain MP-1 represents a novel species of the genus , for which the name sp. nov. is proposed, the type strain is MP-1 (LMG 27927 = MCCC 1K00250).

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Funding
This study was supported by the:
  • Ministry of Science and Technology (Award 2011CB710800 and 2012AA022206C)
© 2014 IUMS
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2014-09-01
2024-04-19
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Burkholderiajiangsuensis sp. nov., a methyl parathion degrading bacterium, isolated from methyl parathion contaminated soil
Int J Syst Evol Microbiol 64, 3247 (2014); https://doi.org/10.1099/ijs.0.064444-0
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