1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_10

sp. nov., isolated from soil Free

Abstract

The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus . On ISP2 medium, strain HM 1154 produced a dark cream, branched substrate mycelium and aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 °C and pH 6–9 with 0–4 % (w/v) NaCl. Whole-cell hydrolysates contained -diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H) and MK-9(H) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C and anteiso-C. Strain HM 1154 exhibited the highest 16S rRNA gene sequence similarities to DSM 40146 (99.4 %), DSM 40346 (99.3 %), DSM 41920 (99.2 %), DSM 40831 (99.2 %), DSM 40260 (99.1 %), DSM 40509 (99.1 %), DSM 40027 (99.1 %), DSM 40029 (99.0 %) and DSM 40037 (99.0 %). DNA–DNA hybridization studies showed relatedness values of 11.0–35.8 % with the closest related species. Based on these results, strain HM 1154 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HM 1154 ( = DSM 42018 = UTMC 1154 = CECT 8305).

  • Published Online:
Funding
This study was supported by the:
  • Biotechnology Development Council of Iran
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.064527-0
2014-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/10/3434.html?itemId=/content/journal/ijsem/10.1099/ijs.0.064527-0&mimeType=html&fmt=ahah

References

  1. Bouizgarne B., Lanoot B., Loqman S., Spröer C., Klenk H.-P., Swings J., Ouhdouch Y. ( 2009 ). Streptomyces marokkonensis sp. nov., isolated from rhizosphere soil of Argania spinosa L.. Int J Syst Evol Microbiol 59, 2857–2863. [View Article] [PubMed]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461–466. [View Article] [PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221–230. [View Article] [PubMed]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  5. Hamedi J., Mohammadipanah F., Klenk H.-P., Pötter G., Schumann P., Spröer C., von Jan M., Kroppenstedt R. M. ( 2010 ). Streptomyces iranensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 60, 1504–1509. [View Article] [PubMed]
    [Google Scholar]
  6. Hamdali H., Virolle M. J., von Jan M., Spröer C., Klenk H.-P. ( 2011 ). Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine. . Int. J. Syst Evol Microbiol 61, 1104–1108. [CrossRef]
    [Google Scholar]
  7. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  8. Kämpfer P. ( 2012 ). Order XIV. Streptomycetales ord. nov.. In Bergey's Manual of Systematic Bacteriology, vol. 5, , 2nd edn., pp 1446–1767. Edited by Whitman W. B., Parte A., Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Ludwig W., Suzuki K. . New York:: Springer;.
     [Google Scholar]
  9. Kawato N., Shinobu R. ( 1959 ). On Streptomyces herbaricolor sp. nov., Supplement: a single technique for microscopical observation. . Mem Osaka Univ Lib Arts Edu B Nat Sci 8, 114–119.
     [Google Scholar]
  10. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  11. Kim B. Y., Zucchi T. D., Fiedler H. P., Goodfellow M. ( 2012 ). Streptomyces cocklensis sp. nov., a dioxamycin-producing actinomycete. . Int J Syst Evol Microbiol 62, 279–283. [View Article] [PubMed]
    [Google Scholar]
  12. Kontro M., Lignell U., Hirvonen M.-R., Nevalainen A. ( 2005 ). pH effects on 10 Streptomyces spp. growth and sporulation depend on nutrients. . Lett Appl Microbiol 41, 32–38. [View Article] [PubMed]
    [Google Scholar]
  13. Kroppenstedt R. M. ( 1982 ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5, 2359–2367. [View Article]
    [Google Scholar]
  14. Kroppenstedt R. M. ( 1985 ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics, pp. 173–179. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  15. Kroppenstedt R. M., Goodfellow M. ( 2006 ). The family Thermomonosporaceae: Actinocorallia, Actinomadura, Spirillospora and Thermomonospora . . In The Prokaryotes, , 3rd edn., vol. 3, pp. 682–724. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  16. Laatsch, H. ( 2011 ). AntiBase 2011: a database for rapid dereplication and structure determination of microbial natural products . Weinheim:: Wiley-VCH;. http://wwwuser.gwdg.de/~hlaatsc/antibase.htm .
  17. Lechevalier M. P., Lechevalier H. A. ( 1970 ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20, 435–443. [View Article]
    [Google Scholar]
  18. Li W., Lanoot B., Zhang Y., Vancanneyt M., Swings J., Liu Z. ( 2002 ). Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. . Int. J. Syst. Evol. Microbiol. 52, 1629–1633. [CrossRef]
    [Google Scholar]
  19. Liu Z., Zhao X., Bai F. ( 2013 ). Production of xylanase by an alkaline-tolerant marine-derived Streptomyces viridochromogenes strain and improvement by ribosome engineering. . Appl Microbiol Biotechnol 97, 4361–4368. [View Article] [PubMed]
    [Google Scholar]
  20. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H. P. ( 2013 ). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195, 413–418. [View Article] [PubMed]
    [Google Scholar]
  21. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  22. Mohammadipanah F., Matasyoh J., Hamedi J., Klenk H. P., Laatsch H. ( 2012 ). Persipeptides A and B, two cyclic peptides from Streptomyces sp. UTMC 1154. . Bioorg Med Chem 20, 335–339. [View Article] [PubMed]
    [Google Scholar]
  23. Montero-Calasanz M. C., Göker M., Pötter G., Rohde M., Spröer C., Schumann P., Gorbushina A. A., Klenk H. P. ( 2013 ). Geodermatophilus africanus sp. nov., a halotolerant actinomycete isolated from Saharan desert sand. . Antonie van Leeuwenhoek 104, 207–216. [View Article] [PubMed]
    [Google Scholar]
  24. Nolan R. D., Cross T. ( 1988 ). Isolation and screening of Actinomycetes. . In Actinomycetes in Biotechnology, pp. 1–8. Edited by Goodfellow M., Williams S. T., Mordarski M. . London:: Academic Press;. [View Article]
    [Google Scholar]
  25. Nonomura H., Ohara Y. ( 1969 ). Distribution of actinomycetes in soil. VI. A culture method effective for both preferential isolation and enumeration of Microbispora and Streptosporangium strains in soil. . J Ferment Technol 47, 463–469.
     [Google Scholar]
  26. Nonomura H., Ohara Y. ( 1971 ). Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes in soil. . J Ferment Technol 49, 895–903.
     [Google Scholar]
  27. Pridham T. G., Hesseltine C. W., Benedict R. G. ( 1958 ). A guide for the classification of streptomycetes according to selected groups; placement of strains in morphological sections. . Appl Microbiol 6, 52–79.[PubMed]
    [Google Scholar]
  28. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. ( 1996 ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46, 1088–1092. [View Article] [PubMed]
    [Google Scholar]
  29. Sasser, M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 . Newark, DE:: MIDI Inc;.
  30. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407–477.[PubMed]
    [Google Scholar]
  31. Schleissner C., Pérez M., Losada A., Rodríguez P., Crespo C., Zúñiga P., Fernández R., Reyes F., de la Calle F. ( 2011 ). Antitumor actinopyranones produced by Streptomyces albus POR-04-15-053 isolated from a marine sediment. . J Nat Prod 74, 1590–1596. [View Article] [PubMed]
    [Google Scholar]
  32. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  33. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226–231.[PubMed]
    [Google Scholar]
  34. Vaas L. A. I., Sikorski J., Michael V., Göker M., Klenk H. P. ( 2012 ). Visualization and curve-parameter estimation strategies for efficient exploration of phenotype microarray kinetics. . PLoS ONE 7, e34846. [View Article] [PubMed]
    [Google Scholar]
  35. Vaas L. A. I., Sikorski J., Hofner B., Fiebig A., Buddruhs N., Klenk H.-P., Göker M. ( 2013 ). opm: an R package for analysing OmniLog(R) phenotype microarray data. . Bioinformatics 29, 1823–1824. [View Article] [PubMed]
    [Google Scholar]
  36. Waksman S. A., Henrici A. T. ( 1943 ). The nomenclature and classification of the Actinomycetes. . J Bacteriol 46, 337–341.[PubMed]
    [Google Scholar]
  37. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  38. Wellington E. M. H., Stackebrandt E., Sanders D., Wolstrup J., Jorgensen N. O. G. ( 1992 ). Taxonomic status of Kitasatosporia, and proposed unification with Streptomyces on the basis of phenotypic and 16S rRNA analysis and emendation of Streptomyces Waksman and Henrici 1943, 339AL . . Int J Syst Bacteriol 42, 156–160. [View Article] [PubMed]
    [Google Scholar]
  39. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 1743–1813.[PubMed]
    [Google Scholar]
  40. Williams S. T., Goodfellow M., Alderson G. ( 1989 ). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T., Sharpe M. E., Holt J. G. . Baltimore:: Williams & Wilkins;.
     [Google Scholar]
  41. Witt D., Stackebrandt E. ( 1990 ). Unification of the genera Streptoverticillum and Streptomyces, and amendation of Streptomyces Waksman and Henrici 1943, 339AL . . Syst Appl Microbiol 13, 361–371. [View Article]
    [Google Scholar]
  42. Zhao G. Z., Li J., Qin S., Huang H. Y., Zhu W. Y., Xu L. H., Li W. J. ( 2010 ). Streptomyces artemisiae sp. nov., isolated from surface-sterilized tissue of Artemisia annua L.. Int J Syst Evol Microbiol 60, 27–32. [View Article] [PubMed]
    [Google Scholar]
  43. Zheng J., Zhang X., Xin Y., Han X., Ni S., Zhang J. ( 2013 ). Streptomyces yaanensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 63, 4719–4723. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.064527-0
Loading
Streptomyces zagrosensis sp. nov., isolated from soil
Int J Syst Evol Microbiol 64, 3434 (2014); https://doi.org/10.1099/ijs.0.064527-0
/content/journal/ijsem/10.1099/ijs.0.064527-0
/content/journal/ijsem/10.1099/ijs.0.064527-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error